| Project/Area Number |
59380026
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Nagoya University |
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Principal Investigator |
SATO Hidemi Professor. School of Science, Nagoya University, 理学部, 教授 (40109260)
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| Co-Investigator(Kenkyū-buntansha) |
YONEDA Mitsuki Professor. Faculty of Science, Kyoto University, 理学部, 教授 (50017183)
IZUTSU Kosaku Professor & Dean, School of Medicine, Mie University, 医学部, 教授 (50024617)
MABUCHI Issei Associate Professor, College of General Education, University of Tokyo, 教養学部, 助教授 (40012520)
HATANO Tadashi Professor, School of Science, Nagoya University, 理学部, 教授 (50022570)
ISHIKAWA Harunori Professor. School of Medicine, Gumma University, 医学部, 教授 (90010058)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥12,200,000 (Direct Cost: ¥12,200,000)
Fiscal Year 1986: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1985: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1984: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | Cell Motility / Cotoskeleton / Molecular bases / 生物物理学的解析 |
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| Research Abstract |
Cell motility in eukaryotes are essentially controlled by the filamentous cytoskeletons, namely actin cables (actomyosin), 10 nm intermediate filaments, and microtubules. In this project, we aimed to reveal the molecular bases and the regulatory mechanism to assemble these cytoskeletons. Results obtained were briefly itemized as follows:
a) Involvement of <Ca^(2+)> for the polymerization of mitotic spindles, and the cyclic changesin intracellular free <Ca^(2+)> level associated with cleavage cycles in echinoderm and Medaka eggs were recorded by the improved video-enhanced-microscopy and anlyzed by image synthesizer. Distribution of the membrane-associated <Ca^(2+)> during the cell division also traced using Chlortetracycline as the molecular probe. b) Molecular mechanism of calmodulin binding proteins were analyzed and the cloning of the Tetrahymena actin gene was suceeded. c) Using anti-dynein <I> antibody, the presence of dynein in the mitotic spindle was examined and proved practical
… More
ly no dynein contribution for anaphase chromosome movement. d) Dynamic aspectsof the protoplasmic streaming and the contractile systems in Physarum, Dichotomosiphon, Acetabularia, Vallisneria, Caulerpa and Chara were carefully examined and some ultrastructural analysis were made to distinguish their cytoskeletal interactions. e) Periodicity of cytoplasmic cycle controlled by build-in biological clock and the mechanical properties of the egg surface under the various experimental conditions were measured. f) Biochemistry of cytokinesis was intensely studied in term of the actin bundle formation, regulatory proteins to assemble or disassemble actin cables and <Ca^(2+)> involvement.
2nd item is the organization of the colloquium on the "Biophysics and Moleculer Aspects on Cell Motility", which was originated by the effort of Drs. Ebashi, Oosawa, Kamiya and Tonomura 30 years ago. We organized this colloquium jointly with other Co-operative Research Grant awardees. Paper presentations were: 115 in 1984, 113 in 1985 and 118 in 1986. This is the unique and interdisciplinary meeting with up-tp-date high standard and widely accepted and evaluated by japanese scientific community. We strongly hope the continuation of this colloquium with the support of the Grant-in-Aids awarded by our government. Less
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