On formation and chemical structure of functional oligasaccharide from lactose

Research Project

Project/Area Number	59430022
Research Category	Grant-in-Aid for General Scientific Research (A)
Allocation Type	Single-year Grants
Research Field	畜産化学
Research Institution	Tohoku University
Principal Investigator	ADACHI Susumu 東北大学, 農, 教授 (10005580)
Project Period (FY)	1984 – 1985
Project Status	Completed (Fiscal Year 1985)
Budget Amount *help	¥21,200,000 (Direct Cost: ¥21,200,000) Fiscal Year 1985: ¥4,500,000 (Direct Cost: ¥4,500,000) Fiscal Year 1984: ¥16,700,000 (Direct Cost: ¥16,700,000)
Keywords	galacto-oligosaccharire / <beta> -galactosidase / ラクトース
Research Abstract	Di-, tri-, tetra-, penta- and hexasaccharides were formed during the hydrolysis of lactose by transgalactosylation reaction of Aspergillus oryzae <beta> -galactosidase. In this study the isolation and characterization of the major constituents of tri-, tetra- and pentasaccharides are described. The structure elucidation of 3 tri-, 2 tetra- and 1 pentasaccharides was carried out by methylation analysis, mass spectrometry and <^(13)C> -nmrspectrometry. The trisaccharides are 3'-galactosyl-lactose, 6'-galactosyl -lactose and 4,6-digalactosyl-glucose. Tetrasaccharides are O- <beta> -D-galacto pyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose and O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-3) [or O- <beta> -D-galactopyranosyl-(1-3)-O- <beta> -D-galactopyranosyl-(1-6)]-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose. Pentasaccharide is O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-6)-O- <beta> -D-galactopyranosyl-(1-4)-D-glucose.