| Project/Area Number |
59430025
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical pharmacy
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
IKEKAWA Nobuo Faculty of Science, Tokyo Institute of Technology, 理学部, 教授 (50016119)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Yuji same as above, 理学部, 助手 (30134810)
INADA Yuji same as above, 理学部, 教授 (40016035)
FUJIMOTO Yoshinori same as above, 理学部, 助手 (50173472)
MORISAKI Masuo same as above, 理学部, 助教授 (30016159)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1984: ¥17,000,000 (Direct Cost: ¥17,000,000)
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| Keywords | Oxygenated sterol / Gaschromatography-mass spectrometry / Micro-analysis / Blood component / Plant sterol / Plasminogen activator / 植物ステロール / 赤血球 / 血液 |
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| Research Abstract |
This study deals with various biological effects of oxygenated sterols on blood components such as erythrocytes and platelets in relation with diseases like atherosclerosis and heart disease, and resulted in the following findings.
(1) Sitosterol and fucosterol were found to produce plasminogen activator in cultured endothelial cells from bovine carotod artery.
(2) Some of the oxygenated sterols found to cause rapid modulation of platelet aggregation in plasma. (22R)-22-Hydroxycholesterol found to specifically undertakes lysis of platelets and erythrocytes through its incorporation into membrane.
(3) Cholesterol analogues with a carbene-generating substituent on the side chain were synthesized and their photochemical reactions were investigated in a model system.
(4) Oxygenated sterols of fungus-infected silkworm carcass, Bombyx cum Botryte were analyzed.
(5) 15-Oxygenated sterols were found to be a potent inhibitor of enzymatic conversion of dihydrolanosterol into cholesterol.
(6) Natural sterols and terpenoids with biological activities related in this project were investigated and their structures were determined.
(7) Serum sterols from patients (hypertension, atherosclerosis) were analyzed for oxygenated sterols.
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