| Project/Area Number |
59430026
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | The University of Tokyo, Faculty of Engineering |
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Principal Investigator |
MIURA Kin-ichiro Professor, Faculty of Engineering, The Univ. of Tokyo, 工学部, 教授 (30000227)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAO Ichiro Assistant, Faculty of Engineering, The Univ. of Tokyo, 工学部, 助手 (50173216)
KUMAGAI Izumi Assistant, Faculty of Engineering, The Univ. of Tokyo, 工学部, 助手 (10161689)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥30,500,000 (Direct Cost: ¥30,500,000)
Fiscal Year 1986: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1985: ¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1984: ¥20,600,000 (Direct Cost: ¥20,600,000)
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| Keywords | protein synthesis / messenger RNA (mRNA) / ribosome / gene expression / eukaryote / 遺伝情報発現の調節 / タンパク貭合成 |
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| Research Abstract |
To study the messenger RNA structure relating on control of the rate of protein synthesis, especially in the initiation step, the method for chemical synthesis of RNA fragment was studied. 11 nucleotides long RNA fragment was obtained by phospho-truester method on solid-phase. The initiation codon A-U-G and ralated sequences were synthesized. Some of them were capped chemically. All the oligonucleotides were added with a radioisotopically labelled nucleotide [5'-^<32>P]pCp to the 3' terminus for detection of small amount of samples. Formation of the initiation complex for protein synthesis in the eukaryotic system was studied with the chemically synthesized oligonucleotides under addition of sparsomycin to prevent chain elongation. With non-capped A-U-G, the initiation complex is formed, though it is very small amount. The cap structure is required to enchance protein synthesizing activity. The leader sequences the rate further depending on its constitution.
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