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Studies on the non-specific immunomodulator effects in animals by treatment of Toxoplasma lysate antigens

Research Project

Project/Area Number 59440017
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionOBIHIRO UNIVERSITY

Principal Investigator

SUZUKI Naoyoshi  Prof., Dept. Vet. Physiol. & Protozoan Immunol.,Obihiro University, 畜産学部, 教授 (10003071)

Co-Investigator(Kenkyū-buntansha) SATO Motoyoshi  Assist.Prof.,Dept. Vet. Clinical Radiology, Obihiro University, 畜産学部獣医放射線学講座, 講師 (50003140)
MIKAMI Masayuki  Assoc.Prof.,Dept. Animal Science, Obihiro University, 畜産学部肉畜保蔵学講座, 助教授 (40003107)
SAITO Atsushi  Assoc.Prof., Dept.Vet.Physiol. & Protozoan Immunol.,Obihiro University, 畜産学部家畜生理学講座, 助教授 (10002263)
MIURA Hiroyuki  Prof.,Dept. Animal Science, Obihiro University, 畜産学部肉畜保蔵学講座, 教授 (90003079)
HIROSE Tsuneo  Prof.,Dept. Vet. Clinical Radiology, Obihiro University, 畜産学部獣医放射線学講座, 教授 (60003076)
Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥25,500,000 (Direct Cost: ¥25,500,000)
Fiscal Year 1986: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1985: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1984: ¥15,500,000 (Direct Cost: ¥15,500,000)
KeywordsLong-term suspension culture / Toxoplasma gondii / Babesia rodhaini / Plasmodium berghei / Immunomodulator / TLA / Immunoregulator / Obioactin / オビオアクチン / BRM / 組織培養 / モジュレーター
Research Abstract

When animals (mice, rats and cattle) were pretreated with Toxoplasma lysate antigens(TLA), lymphokines such as IFN,MAF,MIF,TOXO-GIF appeared in their blood and they showed a strong non-specific resistance. In consequence, in order to clarify the defence mechanisms which can be induced by TLA against various infections, the mass cultivation of Toxoplasma organisms is necessary. Within the research period of this Grant, we established as the lst report in the world, a method of mass and long term propagation of Toxoplasma in suspension cultures for 30-60 days period and also found a technique for separation of the organisms from host cells automatically, using a microcomputer-controlled system. Then, TLA was used to examine the effect of immunomodulator in reducing clinical symptoms of Babesia, Plasmodium or Theileria infection in animals. In the main results obtained in our studies, non of the mice in the non-TLA treated control groups survived for more than 2 weeks after Babesia or Plasmodium infection, however, mice pretreated with TLA alone or together with lymphokiens or Obioactin survived in a high percentage. In the pretreated cattle infected with Theileria, the clinical symptoms and anemia were extremly slight as compared with those of the non-TLA treated cattle. When animals were treated with TLA, Thy-1 and <Lyt^1> positive and <Lyt^2> negative lymphocytes were found to accumulate remarkably in the thymus and spleen and there was a remarkable increase in the immune response of the host to infection with protozoan organisms.

Report

(2 results)
  • 1986 Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] SUZUKI,N.;IZUO,A.;SAKURAI,H.;SAITO,A.;MIURA,H.;OSAKI,H.: Zentralblatt f【u!¨】r Bakteriologie,Mikrobiologie und Hygiene A (International Journal of Microbiology and Hygiene)Zbl.Bakt.Hyg.A. 256. 356-366 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] OMATA,Y.;SAKURIA,H.;IZUMO,M.;SAITO,A.;SUZUKI,N.: Japanese Journal of Veterinary Science Jpn.J.Vet.Sci.46. 155-165 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] OGAWA,R.;SATO,M.;HIROSE,T.;SUZUKI,N.: Jpn.J.Vet.Sci.47. 225-236 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] SATO,M.;HORI,Z.;HIROSE,T.;SUZUKI,N.: Jpn.J.Vet.Sci.47. 921-929 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] SATO,M.;SEKIGUCHI,S.;MIYAGAMI,T.;SAKURAI,H.;SAITO,A.;HIROSE,T.;SUZUKI,N.: Zbl.Bakt.Hyg.A. 258. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] SAKURAI,H.;TEGOSHI,K.;TAKAGI,T.;SAITO,A.;SUZUKI,N.;SEITZ,H.: Zbl.Bakt.Hyg.A. 258. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 鈴木直義著,南哲郎,藤永徹編: "獣医住血微生物病:実験動物のバベシア病,マラリア,トリパノゾーマ病,その他の住血微生物病" 近代出版, 386 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 鈴木直義著,伊沢久夫,清水悠紀臣編: "獣医学 1986:オビオアクチン-OBIOACTIN-生体応答修飾物質(BRM)中の天然免疫調整剤として" 近代出版, (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] SUZUKI,N.;IZUMO,A.;SAKURAI,H.;SAITO,A.;MIURA,H.;OSAKI,H.: "Toxoplasmacidal activity of OBIOACTIN derived from hydrolyzed Toxoplasma immune bovine serum in heterologus cell cultures" Zentralblatt fur Bakteriologie,Mikrobiologie und Hygiene (Zbl.Bakt.Hyg.) A. 256. 356-366 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] OMATA,Y.;SAKURAI,H.;IZUMO,M.;SAITO,A.;SUZUKI,N.: "Interferon production in non-immune and immune mice after Toxoplasma infection" Japanese Journal of Veterinary Science (Jpn. J. Vet. Sci.). 46. 155-165 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] OGAWA,R.;SATO,M.;HIROSE,T.;SUZUKI,N.: "Preventive effect of Toxoplasma lysate antigen (TLA) on fatal infection with mouse piroplasma" Jpn. J. Vet. Sci.47. 225-236 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] SATO,M.;HORI,Z.;HIROSE,T.;SUZUKI,N.: "Immune effect of Toxoplasma lysate antigen on cattle against Theileria sergenti infection" Jpn. J. Vet. Sci.47. 921-929 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] SATO,M.;SEKIGUCHI,S.;MIYAGAMI,T.;SAKURAI,H.;SAITO,A.;HIROSE,T.;SUZUKI,N.: "With time changes of T-lymphocytes after Babesia infection in mice pretreated with Toxoplasma lysate antigen" Zbl. Bakt. Hyg. A. 258. (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] SUZUKI Naoyoshi (Edited by Minami,T.; Fujinaga,T.): Kindai Shuppan Co. Lts., Tokyo. Haemomicrobiological Diseases in Veterinary Medicine ( in Japanese ), 386 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 第5回日独原虫病国際シンポジウム. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] 第5回日独原虫病国際シンポジウム. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] 第5回日独原虫病国際シンポジウム. (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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