| Project/Area Number |
59440041
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
1984 – 1985
|
| Project Status |
Completed (Fiscal Year 1985)
|
| Budget Amount *help |
¥22,500,000 (Direct Cost: ¥22,500,000)
Fiscal Year 1985: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1984: ¥19,000,000 (Direct Cost: ¥19,000,000)
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| Keywords | gene transfer / transfection / transformation / hepatoma / NIH / 3T3 / hepatitis B virus / DNA / クローニング |
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| Research Abstract |
We have studied by means of DNA-mediated gene transfer the transforming activity of the DNA of the human hepatoma cell line HCC-M. The following results have been obtained.
1. HCC-M possesses characteristics of the cell derived from the human hepatoma.
2. It has been demonstrated that HCC-M cells have integrated hepatitis B virus (HBV) DNA although they do not produce HBVassociated antigens.
3. DNA from HCC-M induced transformed foci on transfection of NIH/3T3 cells. DNAs from primary transformants were capable of inducing secondary transformants and DNAs from the secondary transformants were also capable of inducing tertiary transformants.
4. Most of these secondary transformants were demonstrated to contain both human repetitive sequences and HBV DNA, indicating that the transformants had incorporated exogenous human DNA and HBV DNA as well. This result suggests that there is a close relationship between HBV DNA and transforming gene(s) in HCC-M.
5. A 3.5 kd DNA segment was cloned from one of the secondary transformants using pUC13 vector. This segment hybridized with both HBV DNA and v-K-ras genome.
From these results it is suggested that the integration of HBV DNA is fairly closely associated with the activation of oncogene(s) in a human hepatoma cell HCC-M.
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