| Project/Area Number |
59440046
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
YATA Junichi Tokyo Medical and Dental University, Faculty of Medicine, 医学部, 教授 (60057502)
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| Co-Investigator(Kenkyū-buntansha) |
下沢 和彦 東京医科歯科大学, 医学部, 助手 (70154317)
OHKAWA Hiroji Tokyo Medical and DEntal University, Faculty of Medicine, 医学部, 助手 (90160431)
INADA Makoto Tokyo Medical and Dental University, Faculty of Medicine, 医学部, 助手 (20151582)
MATSUOKA Yoshiko Tokyo Medical and Dental University, Faculty of Medicine, 医学部, 助手 (90134676)
KABASAWA Yasuhiro Tokyo Medical and Dental University, Faculty of Medicine, 医学部, 講師 (40014279)
SHIMOZAWA Kazuhiko Tokyo Medical and DEntal University, Faculty of Medicine
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1984: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | Immunodeficiency / Chronic thyroiditis / Myasthenia gravis / Autoantibody / Suppressor T cell / Hybridoma / 免疫調節 / 自己抗体 / 慢性甲状腺炎 / 重症筋無力症 / ヘルパーT細胞 / T細胞抗原 |
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| Research Abstract |
In order to analyse the immunoreguratory system involved in the pathogenesis of immune disorders, human lymphocytes from normals, immunodeficiency patients and autoimmune diseases were studied in vitro.
1. An experimental system to analyse antibody production from the lymphocytes stimulated by specific antigens such as ovalbumin,tetanus toxoid,thyroglobulin and acetylcholine-receptor was established.
2. At least two distinct T cell subsets were proved to be involved for the regulation of antibody formation and both antigen specific and nonspecific suppressor factors were secreted from T cells.
3. T cells from healthy individuals suppressed the autoantibody formation from the lymphocytes of chronic thyroiditis or myasthenia gravis.
4. In stimulation with excess dose of thyroglobulin. T cells from healthy individuals secreted humoral factors suppressing anti-thyroglobulin antibody formation from the lymphocytes of chronic thyroiditis.
5. Such T cells were fused with an established T cell leukemia cell line. Thus obtained hybridomas were cloned and one clone which secretes antigen specific suppressor humoral factor for anti-thyroglobulin antibody formation was obtained.
6. Humoral factors which induce suppressor T cells were demonstrated in the sera from cancer patients. Alpha-fetoprotein also induced suppressor T cells.
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