| Project/Area Number |
59440070
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Tohoku University |
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Principal Investigator |
OKUDA Tomoko (1985) 東北大学, 医, 教授 (50091666)
河本 和友 東北大学, 医学部, 教授
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| Project Period (FY) |
1984 – 1985
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| Project Status |
Completed (Fiscal Year 1985)
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| Budget Amount *help |
¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1984: ¥8,500,000 (Direct Cost: ¥8,500,000)
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| Keywords | immune complex / experimental otitis media with effusion / mucociliary transport system / 粘液繊毛運送機能 / 好中球、マクロファージ |
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| Research Abstract |
To investigate the immunological changes of the tubotympanum in the recurrent otitis media, experimental otitis media with effusion was induced by immune complex injection to the bullae of experimental animals. Horseradish peroxidase (HRP) was chosen as an antigen because of its high antigenicity and traceability under light and electron microscopy. Seventy-three guinea pigs and 41 chinchillas were divided into three groups:active Arthus group, passive Arthus group and control group. Animals were killed under general anesthesia from the first to 30th day after the injection. Within 5 days after injection, effusions were observed consistently in all animals, but in only 80 per cent on the 7th day and in only 50 per cent after the 10th day after injection. The most conspicuous findings were that the interciliary space of the mucociliary system was diffusely occupied by an electron-dense mucus, and the upper part of the ciliary shafts were tightly glued together. Immuno-chemical analyses of the effusions also revealed that compliment system of the middle ear mucosa was activated through the alternative pathway.
This study strongly suggested that the various inflammatory products contained in the middle ear effusions elicit a persistent vicious cycle of inflammatory reactions in the tubotympanum.
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