| Project/Area Number |
59470100
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Nagoya University |
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Principal Investigator |
SUGIMOTO Etsuro Nagoya University, Faculty of Agriculture, Professor, 農学部, 教授 (50026522)
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| Co-Investigator(Kenkyū-buntansha) |
KITAGAWA Yasuo Nagoya University, Faculty of Agriculture, Research Associate, 農学部, 助手 (50101168)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1986: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1985: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1984: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | Nutrient / Physiological role / Differenciation / Cultured animal cell / Carcinama / Adipocyte / Extracellular matrix / ビタミンA |
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| Research Abstract |
In order to clarify physiological function of nutrients, two cell lines have been selected as model systems.
Embryonal carcinoma F9 is an interesting clonal cell line because its differenciation is generated by retinoic acid. The effect of retinoic acid and cAMP on the differenciation of F9 cells were studied mainly using the biosynthesis and secretion of laminin as markers. In contrast to the irreversible effect of retinoic acid, the effect of cAMP on F9 differenciation was reversible. Michanisms of post-translational assembly and glycosylation of laminin subunits in F9 cells were clarified. It was suggested that different concentration range of retinoic acid induced differenciation of F9 stem cells into two type cells.
Murine fibroblast 3T1-L1 cells have capability to differenciate from preadipocytes into adipocytes in culture. The endogenous protein phosphorylation stimulated by catecholamines was compared in 3T3-L1 preadipocytes and adipocytes. Stimulated phosphorylation of proteins with molecular weight 90,000, 62,000, 48,000 and 32,000 was observed only in 3T3-L1 adipocytes. The phosphorylation of the 62,000 dalton protein in 3T3-L1 was observed 1 min after the addition of norepinephrine and dephosphorylation was observed within 10 min after the addition of propranolol. Inducer (dexamethasone, 1-methyl-3-isobutylxanthine and insulin)-stimulated adipose conversion of 3To-L1 cells was inhibited by LiCl at concentrations ranzing from 2 to 20 mM. Effect of LiCl was reversible.
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