Cloning and sequencing of a cDNA for poly(A)+RNA responsible for pole cell formation in Drosophila embryos.
| Project/Area Number |
59480018
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Institute of Biological Sciences, University of Tsukuba |
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Principal Investigator |
OKADA Masukichi Professor, Inst. Biol. Sci., Univ. Tsukuba, 生物科学系, 教授 (60015534)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1986: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1984: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | Drosophila / germ cell / pole cell / cytoplasmic determinant / gene cloning / cDNA / rRNA / rRNA / ミトコンドリア / 生殖系列 / CDNA / ミトコンドリアrRNA |
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| Research Abstract |
Final goal of the present project is to elecidate the molecular basis for the function of polar plasm as the germ plasm. In Drosophila, polar plasm is known to autonomously initiate pole cell formation and commit the pole cells to develop to germ cells. We already showed in Drosophila that a maternally transcribed poly(A)^+RNA was responsible for pole cell formation, adn proposed that this RNA was a component of germ plasm. In this project we cloned cDNA of this poly(A)^+RNA, and determined the nucleotide sequence of the cDNA. A search in a DNA data base by a computer program showed that this cDNA sequence is strictly homologous with mitochondrial large ribosomal DNA but not with nuclear DNA.
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Report
(2 results)
Research Products
(13 results)
