| Project/Area Number |
59480035
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
園芸・造園学
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| Research Institution | Kyoto University |
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Principal Investigator |
SUGIURA Akira Faculty of Agriculture, Professor, 農学部, 教授 (00026379)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1984: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | Japanese persimmon / removability of astringency / seed / ethanol, / cultivar differentiation / アイソザイム |
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| Research Abstract |
Japanese persimmon cultivars are classified into 4 different types depending on the ways or degrees their fruits lose astringency on the tree. This study was undertaken to search for the relationships between removability of astringency and cultivar differentiation in terms of ethanol production by seeds and isozyme patterns of pollen and mature leaves.
1). Ethanol production by seeds generally showed 2 peaked curves and the first peak coincided with the rapid growth of embryos. But no difference in ethanol production was observed between seed parts. Alcohol dehydrogenase (ADH) activity in the seed also increased toward the onset of ethanol production. Stratified seeds of pollination constant types tended to show higher ADH activity than pollination-variant ones.
2). Ehanol production was determined with fruits of F_<12> progenies obtained from crosses between pollination-constant and nonastringent (PCNA) types. Progenies having cv. Fuyu as a parent showed wide variation in ethanol production while those otherwise constantly produced low ethanol, suggesting the existence of gene(s) governing ethanol production in the seed.
3). Similarity indices were calculated using 68 bands obtained from 9 pollen isozymes of 19 cultivars. Higher similarities were found among PCNA cultivars. 18 out of 163 cultivars were identified by combining 2 isozyme systems, glucosephosphate isomerase and malate dehydrogenase, of mature leaves. No relationship, however, was dound among the 4 fruit types.
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