| Project/Area Number |
59480048
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
蚕糸学
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| Research Institution | Shinshu University |
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Principal Investigator |
YANAGISAWA YUKIO Faculty of Textile Science and Technology,Shinshu University, 繊維学部, 助教授 (70021160)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1984: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | Cytokinin / IAA / HPTLC / HPLC / Bioassay / Mulberry / Retained leaves / 腋芽 |
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| Research Abstract |
Endogenous cytokinins and IAA (indole-3-acetic acid) in mulberry leaves and shoot tips were separated and identified by several assay methods:HPTLC, HPLC, PVP column and bioassay. 1. Separation and identification of the cytokinins in mulberry.
(1) In extracts of mulberry leaves three substances were separated by HPTLC and further assayed by the 265nm reflection scan of Shimazu CS-930. One of these substances was considered to be ZR, as its chromatographic pattern was similar to that of authentic ZR.
(2) Cytokinin level in the axillary bud on the branch which had elongated after the summer pruning in the previous year was higher than that in the axillary bud on the stump of the branch base which had been formed by spring pruning and both cytokinin levels increased withe the sprouting. 2. Changes in content of IAA in mulberry. (1) It was noted that in both earlier and later stages of growth the IAA content in shoot tip was nearly equal in quantity. (2) In elongation stage (July) the shoot tip had more IAA in comparison with the largest glossy leaf. 3. Relationship between growth of axillary bud on the remaining branch and changes in content of cytokinin in retained leaves after intermediate-cutting in mulberry. (1) Detected cytokinins in retained leaves were three substances of Z, ZR, and 2iP. (2) Z content in retained leaves showed a decreasing tendency with recovery of the physiological function. (3) ZR content in ones increased with the growth from start of regeneration growth of axillary bud to growth of the Dappo and Enko stages. (4) Whether the leaves retain or not the cytokinin in the axillary bud of the same growth became equivalent levels.
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