| Project/Area Number |
59480057
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
発酵・醸造
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OISHI Kunio Associate Professor, Inst. Appl. Microbiol., Univ. Tokyo, 応用微生物研究所, 助教授 (90013317)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOTA Takao Research Associate, Fac. Agr., Univ. Tokyo, 農学部, 助手 (40011986)
SUGIYAMA Junta Associate Professor, Inst. Appl. Microbiol., Univ. Tokyo, 応用微生物研究所, 助教授 (80142256)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1984: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Brassinolide / Oleic acid / Monoolein / Fungi / Plant hormone / イネラミナジョイントテスト |
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| Research Abstract |
Brassinolide activity was screened in methanol extracts of the cells of 1098 fungal strains, accordind to rice lamina inclination test. Thirty two strains were found to show as high as 10 ng/g wet cell-level activity. They belong to Cunninghamella, Penicillium, Trichoderma, Aspergillus, Cladosporium and others. Productivity of brassinolide activity was present prejudically among fungal genera, but commonly in the genus. The activity per cells did not depend on the composition of culture media even when various steroidal compounds were added. The activity in methanol extracts was separated mainly into two fractions in silica gel column chromatography; one which was more hydrophobic than brassinolide and the other which was markedly hydrophilic. The former one was the major substance, weighing 60% of the total active fractions. Four and a half g of the hydrophobic substance was purified from 7 kg wet cells of Cunninghamella blakesleeana by successive extraction with methanol, ethyl acetate, and acetone, column chromatographys on almina and silica gel, and preparative HPLC. The substance was identified by TLC and GC/MS as monoolein. Commercial products of monoolein from plants also showed brassinolide activity. Activity of monoolein was one twenty thousandth of brassinolide and was detected at a level of 10 <micro> g/ml. Oleic acid was tenth as active as monoolein. Physiological role of oleic acid and its derivatives in plant was discussed.
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