Genetic analysis of mtDNA in marine organisms based on restriction endonuclease cleavage patterns.
| Project/Area Number |
59480067
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Otsuchi Marine Research Center, Ocean Research Institute University of Tokyo. |
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Principal Investigator |
NUMACHI Ken-ichi Prof., Otsuchi Mar. Res. Cent.,Ocean Res.Inst.,Univ.of Tokyo., 海洋研究所, 教授 (30013569)
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| Co-Investigator(Kenkyū-buntansha) |
沼知 健一 東京大学海洋研究所, 大槌臨海研究センター, 教授 (30013569)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1984: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Mitochondrial DNA / Restriction enzyme pattern / Fishes / 有用海産動物 |
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| Research Abstract |
Genetic studies on marine organisms much progressed by applying immunogenetical method in 1950, and by isozyme analysis from 1960. Isozyme analysis have contributed to accumulate many knowlege in a wide variety of biology, such as enzymology, embryology, physiology, evolution, and population genetics. However, identification of population in marine organisms, which is regarded as unit of stock, is not always possible even by isozyme analysis. Thus, new genetic tags were to be found out for population analysis. This study is aimed to establish analytical method of mtDNA in marine organisms and make clear the usefulness of restriction fragment patterns of mtDNA as a genetic tag in marine organisms.
Mitochondria of fish species are apt to be ruptured compared with mammals including dolphins, and mtDNA was decomposed by DNase treatment to mitochondoria pelets. Then, nuclear DNA were segimented by increment of salt concentration in SDS solution. A small quantity of crude mtDNA or mtDNA samples containing nuclear DNA were showed to get clear segment patterns by applying Southern blot hybridization.
mtDNA was purified in dolphins, salmonids, Japanese dace and oyster species in a scale of population study. Of these Japanese dace were most extensively studied. The results of this species, showed that ( 1 ) the number of base pair is 16.3Kbp as in human and other organisms so far studied,( 2 ) polymorphisms were found with eight enzymes of ten of 6 base recognition enzymes, seven genomes were discriminated in Otsuchi Bay population and Monobe River population, each genotypes was population specific and interpopulational divergence was estimated to be a 1%. Thus, analytical method of the restriction fragment patterns of mtDNA and its usefulness in population studies of marine organisms should be shown in this project.
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Report
(1 results)
Research Products
(4 results)
