| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1985: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1984: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Research Abstract |
A similarity between feline panleukopenia (FPL), mink enteritis (ME) and canine parvoviruses (CP) in virological and serological properties was described by many investigators. While some differences among the viruses were demonstrated on the biological property. Especially, CPV infection has a wide distribution throughout the world since the year 1978, and this infection has received special attention as a new canine virus disease. This paper concerns the comparative studies of biological and molecular biological properties among the viruses.
Mev had about the samw properties as FPLV in cross-HI tests, in the propagating ability of viruses in MDCK calls and in the hemagglutinability, whereas the heat stability at 80゜C, and the restriction enzyme digestion patterns of the viral replicating form (RF) DNAs were similar to those of CPV. The nucleotide sequence of cloned MEV RF-DNA was analysed and the gene organization was determined. The sequence determined was 3,510 nucleotides long, which shared 70% of whole MEV DNA.In the DNA homology, and in the amino acid sequence, among MEV, FPLV and CPV, MEV showed slightly higher homology with FPLV than CPV. The cloned DNA was introduced into a feline kidney cell line (CRFK), by transfection, and expression of the DNA in the cells was examined.Intranuclear inclusion bodies, which resembled to those found in the MEV-infected cell nuclei, were observed in the transfected cells 48 hours post transfection (p.t.). Viral capsid proteins VP-2' and VP-2 were also detected in the cells. However, it was failed to recover infectious MEV virions from the transfected cell cultures havested for 4 to 7 days p.t. These results indicate that the cloned MEV RF-DNA has been able to express at least some of its functions in CRFK cells.
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