| Project/Area Number |
59480084
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎獣医学
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| Research Institution | University of Tokyo |
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Principal Investigator |
FUJIWARA Kosaku Faculty of Agriculture, University of Tokyo, 農学部, 教授 (60012689)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMURA Seiji Faculty of Agriculture, University of Tokyo, 農学部, 助手 (10161366)
TAKAHASHI Reiji Faculty of Agriculture, University of Tokyo, 農学部, 助教授 (80011998)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1984: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Antigenic relatedness / Bacillus piliformis / Immunofluorescence / Immunoperoxydase / Monoclonal antibody / Serology / 免疫螢光法 |
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| Research Abstract |
Serologic studies on Tyzzer's disease world-wide spreading in various species of animal were made and the following results were obtained:
1) By complement fixation, gel diffusion, immunofluorescence as well as mouse protection tests using mouse or rat antisera and microbial antigens from infected mouse liver, close antigenic relatedness was revealed between a mouse strain from Denmark and that from Japan, between these murine strains and rabbit strains from America and West Germany as well as of a Mongolian gerbil strain from West Germany. The hamster and kitten strains from Japan, however, were antigenically far from the murine or rabbit strains. 2) Enzyme-linked immunosorbent assay (ELISA) revealed a good correlation on titers between immunofluorescence and ELISA, which was shown to be useful for studying antigenic relatedness among different bacterial strains. 3) Two monoclonal antibodies (MAb) to the RT strain of rat origin, were established, and one of the IgG2a MAb recognized an bacterial antigen having a molecular weight (M.W.) of 54,000 and combined with the bacterial flagella, while the other IgM recognized another antigen with M.W. of 73,000 and combined with the bacterial cell surface. Both MAb were protective for the challenge infection in mice in mouse hepatocyte culture. 4) Using immunoperoxidase method microbial antigen was first detected on Day 2 postinoculation (p.i.) within very small necrotized foci. When necrotic foci increased in size and number, many immunoperoxidase- positive bacteria were present within seemingly living hepatocytes at the periphery of lesions but not within the necrotized foci. Apart from large- sized necrotized foci some hepatocytes carrying abundant bacterial antigen were singly present without inducing any mesenchymal response. Electron microscopy revealed that antibody specifically combined with the flagella and bacterial surface.
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