Study on the central mechanism for gonadotropin secretion in the female rat by brain transplantation technique.
| Project/Area Number |
59480114
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
環境生理学(含体力医学･栄養生理学)
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| Research Institution | Yokohama City University |
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Principal Investigator |
TANAKA Fukuko Professor Yokohama City University School of Medicine, 医学部, 教授 (40046066)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIHARA Masugi Lecturer Yokohama City University School of Medicine, 医学部, 助手 (90145673)
ARITA Jun Assist. Prof. Yokohama City University School of Medicine, 医学部, 講師 (80128587)
AKEMA Tatsuo Assist. Pror. Yokohama City University School of Medicine, 医学部, 講師 (60128585)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1984: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | Brain Transplantation / Dopamine Neuron / Noradrenaline NeuronSubstantia Nigra,Locus Coeruleus / Third Ventricle / Rat Estrous Cycle / Gonadotropin Secretion / Blood Pressure / 卵巣ホルモン正フィードバック / ドーパミン細胞 / ノルアドレナリン細胞 / 視索前野 / ラット膣周期 |
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| Research Abstract |
1. Method for transplantation of catecholamine-neuron in the brain: The neural tissue including A-6 group noradrenaline (NA)-neurons in the locus coeruleus (for NA-rich transplant) or A-10 group doapmine (DA)-neurons in the substantia nigra (for DA-rich transplant) was punched out of the coronal slice dissected from the medulla or midbrain of newborn rats, respectively, with a metal cannula and was stereotaxically positioned in the third ventricle at the level of the preoptic region of cycling rats. Surviving transplant was confirmed to have catecholaine neurons in the Falck-Hillarp fluorescence method.
2. Reproductive function of the female rats with catecholamine-neuron transplants in the third ventricle: The female rat that had surviving NA-neuron rich transplant frequently showed prolongation of estrus, indicating that ovulation was severely impaired. However, after ovariectomy, they showed a pulsatile secretion of LH with a remarkably large amplitude. The DA-neuron rich transplants
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sustained the estrous cycle unchanged, and did not change pulsatile secretion of LH after ovariectomy, too. The results suggested that the NA-neurons rich transplants at the preoptic region somehow inhibited gonadotropin secretion in the intact rat, whereas they facilitated it in ovariectomized rats.
3. Blood pressure of the rats with catecholamine-neuron transplant in the third ventricle: The rats with either transplant, NA-neurone ro DA-neuron, showed a long lasting hypotension. The hypotensive effect was exerted even on the hypertension produced by salt-loading.
4. Effect of estrogen-progesterone treatment on the gonadotropin secretion in the gonadectomized male rat with the ventricular implantation of the preoptic suprachiasmatic nucleus from the female newborn: The preoptic suprachiasmatic nucleus has been considered as the key locus for positive feedback action of progesterone on gonadotropin secretion in the female rat. Transplantation of such a neural tissue in the male brain was not effective to induce LH surge, while FSH secretion was induced. Less
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Report
(2 results)
Research Products
(10 results)
