| Project/Area Number |
59480120
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KURIYAMA Kinya Kyoto Prefectural University of Medicine, Professor, 医学部, 教授 (20079734)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Jun-ichi Kyoto Prefectural University of Medicine, Instructor, 医学部, 助手 (50188132)
OHKUMA Seitaro Kyoto Prefectural University of Medicine, Associate Professor, 医学部, 助教授 (30152086)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1984: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | GABA receptor / Benzodiazepine Receptor / C1 Channel / Functional Coupling / Functional Reconstitution / Receptor Antibody / Immunohistochemistry / Distribution in Brain / Glycoprotein / クロライドチャンネル / 機能的共役 |
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| Research Abstract |
Purification of solubilized -aminobutyric acid (GABA) and benzodiazepine (BZP) receptors from the bovine cerebral cortex was exployes by the affinity columu chromatography using a benzo-diazepine, 1012-s, as immobilized ligand. The gel filtration using Sephadex G-200 of the pufified GABA/BZP receptor complex revealed the molecular weight of the receptor complex was approximately 340,000. SDS-polyacrylamide gel electrophoretic profiles of the 1012-S-eluted fraction showed the existence of two major bands having the molecular weights of approximately 53,000 and 57,000, both of which were irreversibly photoaffinity-labeled with [^3H]flunitrazepam. The purified gaba receptor was found to be a glycoprotein and its carbohydrate chain of a hybrid type was involved in the functional coupling between GABA receptor and BZP receptor. The GABA receptor antibody formed by biweekly subcutaneous injection of the purified GABA receptor to Albino rabbits has revealed immunohistochemically that this receptor complex in the rat brain is localized various brain areas such as the somata of Purkinje cells and gramule cells layer of the cerebellum, large cells in the pars compacta of substantia nigra, pyramidal cells in the cerebral cortex (layeers III-V) and hippocampus, respectively.
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