| Project/Area Number |
59480127
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tsukuba |
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Principal Investigator |
SUGITA Yoshiki The University of Tsukuba,Institute of Basic Medical Sciences, 基礎医学系, 教授 (90019539)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Kiyoshi The University of Tsukuba, College of Medical Technology, 医療技術短期大学部, 教授 (00014142)
KIMURA Sadao The University of Ysukuba, Institute of Basic Medical Sciences, 基礎医学系, 構師 (40134225)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1986: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1984: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Cytochrome <b_5> / cDNA sequences for rabbit cytochrome <b_5> / タンパク質の蔵器特異性 |
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| Research Abstract |
Liver cytochrome <b_5> is a membrane protein consisting of a hydrophilic catalitic region and a hydrophobic region buried in microsomes. Cytochrome <b_5> in erythrocytes is present in soluble fraction and is similar in molecular structure to the hydrophilic domain of the liver enzyme. The two forms of the cytochrome play different functions in different tissues. (1) We have determined the complete amino acid sequence for human, porcine and bovine erythrocyte cytochrome <b_5> , and the sequences of the N-terminal region and joint region of the liver proteins. Of the 97 amino acid residues of erythrocyte cytochrome <b_5> , residues 1-96 were identical with those of the liver proteins of the same species. However, residue 97 (C-terminal residue) was different between the two types, indicating that the two forms of cytochrome <b_5> are encoded by two different but closely related mRNAs. (2) The cDNA encoding rabbit liver cytochrome <b_5> was constructed from mRNA, isolated and sequenced. The cDNA was found to be 727 nucleotides in length. The 5' untranslated region containd 22 bases and was followed by 402 bases of an open reading frame, 286 bases of 3' untranslated region and a poly (A). The 5' side of N-terminal amino acid residue was ATG indicating that the cytochrome had no hydrophobic signal peptide. (3) Using the cDNA of rabbit cytochrome <b_5> as a probe, genomic gene for the human cytochrome <b_5> has been isolated. The sequencing of the gene is now under progress. Although the homology in amino acid residues between human and rabbit cytochrome <b_5> is more than 90%, only less than 50% of the bases at the 3rd podition of the codons of the comparable amino acid residues were identical.
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