| Project/Area Number |
59480152
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Kumamoto University Medical School |
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Principal Investigator |
MASARU Yoshinaga Professor, Kumamoto University Medical School, 医学部, 教授 (90040196)
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| Co-Investigator(Kenkyū-buntansha) |
SUSUMU Ohkawara Kumamoto University Medical School, 医学部, 助手 (10094088)
HIDENORI Takahashi Research Fellow, Kumamoto University Medical School, 医学部, 助手 (40171516)
SHUJI Nakamura Instructor, Kumamoto University Medical School, 医学部, 講師 (90109653)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1986: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1984: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | Inflammation / Polymorphonuclear leukocytes / Interleukin 1 / 細胞運動 / 貧食 |
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| Research Abstract |
During the course of casein-induced inflammation, we measured integration values for the accumulation of polymorphonuclear leukocytes (PMN) and calculated differential calculus for each values. Based on the result, we assumed that emigration of the PMN into the inflammatory site may occur under two different modes, i.e. very rapid emigration at an early stage (1 to 6 hrs) and slow emigration during a later stage (12 to 24 hrs). Then, we measured directly the dx/dt values for the PMN emigration by injecting <^(51)Cr> -labeled PMN i.v. at appropriate intervals of the ongoing inflammation. It was confirmed that there were two different modes of the influx of <^(51)Cr> -PMN, i.e. rapid influx during the early and slow influx during the later, stages of the inflammation.
Next, we compared the PMNs from the early and the later stage of the inflammation in terms of their random motility in vitro. The motility of the early PMN was obviously greater than that of the later PMN. This difference of
… More
PMN in their motility well coincided with the rapid and slow influx of the PMNs in vivo, respectively.
Our earlier studies suggested that peritoneally exudate cells produced an immune potentiation factor during the early stage of inflammation, but not in the later stage. Therefore, we investigated a possibility that PMN may have a functional difference in terms of the production of the factor according to the elapsed time during inflammation. We found that the early PMN had the immune potentiation factor in their cytoplasm, while the later PMN did not.
Furthermore, we purified the immune potentiation factor ultimately and determined its partial aminoacid sequence. The whole aminoacid sequence of the factor was deduced by determining cDNA sequence. The amino acid sequence predicted from the cDNA structure showed a close homology of this substance with interleukin 1 beta of human. Then, we studied the expression of mRNA for the rabbit IL 1 beta by using <^(32)P> -labeled cDNA and found that the expression of the IL 1 beta mRNA was highest at 2 hr after inflammatory stimuli and almost disappeared during 8 to 12 hrs. Thus, we found that the functional difference could be detected in the level of the expression of mRNA. This finding seems to develop a new field for assessing the functional to be devoid of, or very limited in, the capability of protein synthesis. Less
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