Pathogenetic relationship between spontaneous developments of thymoma and muscle abnormality in Buffalo/Mna rats.
| Project/Area Number |
59480155
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
MATSUYAMA Mutsushi Aichi Cancer Center Research Institute, Chief, その他, その他 (80073112)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Minoru Nagoya City University of Pharmacy School, Professor, 藥学部・藥品作用学教室, 教授 (50012638)
NISHI Yoshimi Aichi Cancer Center Research Institute, Research Fellow, 研究所・超微形態学部, 研究員 (50073101)
MASUDA Akira Aichi Cancer Center Research Institute, Research Fellow, 研究所・超微形態学部, 研究員 (50157202)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1984: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Rat of the Buffalo / Mna strain / Spontaneous thymoma / Dominant inheritance / Atrophy of fast twitch muscles / Effects of the introduction of athymic nude gene / Effects of neonatal thymectomy / Epithelial cell lines of rat thymus and thymoma / 培養液へのデキサメサゾン添加効果 |
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| Research Abstract |
We found previously that Buffalo/Mna rats developed spontaneous thymoma, muscle atrophy and glomerulosclerosis at nearly 100%. The present genetic study using crosses between Buffalo/Mna and ACI rats has shown that thymoma susceptibility was determined by a single autosomal dominant gene Tbm-1. The relationships between developments of thymoma, muscle atrophy and glomerulosclerosis were analysed, by using of neonatally thymectomized Buffalo/Mna rats or Buffalo/Mna-rnu/+ rats introduced with athymic nude gene of the rat (rnu). Three day thymectomy produced no effect on the developments of muscle strophy and glomerulosclerosis. In heterozygous Buffalo/Mna-rnu/+ rats, the developments of thymoma and glomerulosclerosis were extremely inhibited, but the development of muscle atrophy was not, compared to littermate Buffalo/Mna-+/+ rats. These results reveal that muscle atrophy of Buffalo/Mna rats is not an associated syndrome with thymoma development, but it is a completely independent abnor
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mality. The inhibitory mechanisms of rnu gene against the thymoma development and the linkage between the developments of thymoma and glomerulosclerosis will be the subjects for future studies.
Cell biological examinations have been performed to elucidate the nature of thymoma. Thymoma epithelial cells of primary cultures had larger cytoplasm, about 2 times as large as thymus epihtelial cells, and secreted a polypeptide of molecular weight about 10,000, which can induce non-specific proliferation of thymic lymphocytes, showing a characteristic of functioning tumor cells. Long-term cultures of thymus and thymoma epithelial cells have been difficult, but we have found that these epithelial cells can proliferate steadily, showing a polygonal shape, in a medium containing 1 <maicro> M dexamethasone. Six thymus and 6 thymoma epithelial cell lines were established as clonal cell lines. We will perform molecular biological analyses on thymoma, using these cell lines and cooperating with molecular biologists. Less
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Research Products
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