| Project/Area Number |
59480163
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | The Institute of Medical Science, The University of Tokyo (1985-1986)
Osaka University (1984) |
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Principal Investigator |
TAKEDA Yoshifumi The Institute of Medical Science, The University of Tokyo, 医科学研究所, 教授 (30029772)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAYAMA Toshiya Thi Institute of Medical Science, The University of Tokyo, 医科学研究所, 助教授 (50050696)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1984: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | Campylobacter Jejuni / Pathogenicity / Cholera-like toxin / 細胞致死毒素 / 高感度ELISA法 |
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| Research Abstract |
1. By means of a newly developed medium, cholera-like enterotoxin production by Campylobacter jejuni could be shown in 25 C. jejuni strains isolated from diarrheic cases. This new medium was found to yield a higher amount of enterotoxin than the two previously reported media for this purpose. Neutralization of the activity of the toxin to cause morphological changes of Chinese hamster ovary (CHO) cells by antisera against cholera toxin and heat-labile enterotoxins of Escherichia coli ( <LT_h> and <LT_p> ) was also demonstrated, indicating a close immunological relation of these toxins.
2. The cytotoxicity of culture filtrates of C. jejuni/coli on CHO cells was also demonstrated with 33 out of 39 C. jejuni and with all 8 C. coli strains.
3. Since the cytotoxic activity of the toxin produced by C. jejuni/coli was neutralized by an antiserum against purified Shiga toxin, we purified Shiga and Shiga-like toxins from Shigella dysenteriae 1 and Escherichia coli 0157:H7 to compare the structures.
4. We developed a highly sensitive ELISA to detect another bacterial toxins as well as the toxins of Campyrobacter. Sensitivity of this ELISA was 200 pg/ml for heat-labile enterotoxin of enterotoxigenic Escherichia coli, 60 pg/ml for Shiga toxin of Shigella dysenteriae 1, 20 pg/ml for a Vero toxin of enterohemorragic E. coli, 6 pg/ml for thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus, and 60 pg/ml for cholera enterotoxin (CT) of V. cholerae.
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