| Project/Area Number |
59480169
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Institute of Medical Science, The University of Tokyo. |
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Principal Investigator |
SHIBUTA Hiroshi Institute of Medical Science, The University of Tokyo, Professor., 医科学研究所, 教授 (70012721)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Kentarou Tokyo Metropolitan Institute of Medical Science, Director., 部長 (90009986)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1984: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Paramyxovirus / Parainfluenza virus / Sendai virus / Genome organization / cDNAクローニング |
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| Research Abstract |
We elucidated completely the genome organization of Sendai virus, a prototype of paramyxovirus group, by analysing complementary DNA clones constructed from the genome RNA. We also analysed genomes of other paramyxoviruses, Newcastle disease virus and bovine parainfluenza 3 virus. Comparison of these genomes characterized the primary structures of viral proteins of the virus group, on one hand, and revealed, on the other hand, the presence of nucleotide sequences which may be signals recognized by viral RNA polymerase. We developed an in vitro transcription system of Sendai virus by introducing a fraction of host cell lysates into the reaction mixtures, and we are now studying roles of the signal sequences mentioned above by using this newly developed transcription system. We also prompted molecular analysis of mutations affecting the pathogenicity of bovine parainfluenza 3 virus by comparing genes of the pathogenic variants of the virus.
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