STUDIES OF PATHOGENETIC MECHANISMS OF IMMUNOLOGICAL ABNORMALITIES IN COLLAGEN DISEASES USING CLONED LYMPHOID LINES
Project/Area Number |
59480202
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Research Category |
Grant-in-Aid for General Scientific Research (B)
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Allocation Type | Single-year Grants |
Research Field |
内科学一般
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Research Institution | JICHI MEDICAL SCHOOL |
Principal Investigator |
KANO SHOGO JICHI MEDICAL SCHOOL, 医学部, 教授 (00049024)
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Project Period (FY) |
1984 – 1986
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Project Status |
Completed (Fiscal Year 1986)
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Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1984: ¥4,800,000 (Direct Cost: ¥4,800,000)
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Keywords | Natural killer cells / Interleukin-2 / Interferon / Cloned lymphocytes: Sjogren's syndrome / Large granular lymphocytes |
Research Abstract |
1.A large granular lymphocyte cell line (PEC-1) with natural killer (NK) cell activity had been established from peritoneal exudate cells, by co-culturing with interleukin 2 (IL-2). Several NK cloned were derived from PEC-1 by limiting dilution. Normal bone marrow cells and thymocytes, as well as NK-sensitive tumor target cell lines were variably killed by cloned NK lines. 2. An IL-2 dependent NK clone produced upon stimulation with PMA five hemopoietic stimulators (BPA, Ep, G/M-CSF, MK-CSF, Eo-CSF), IL-2 and interleukin-3. 3. IL-2 was necessary, but not sufficient, for the continuous growth of NK clones. Macrophages were required for the induction and maintenance of high-affinity IL-2 receptors. The role of macrophages was partially replaced by interleukin-1. 4. T cell receptor (TCR) genes were rearranged and transcribed in cloned LGL lines. However, since the rearrangement pattern of beta-chain genes did not correlated with the cytotoxic activity of NK clones, the cytotoxic activity may not be directly mediated by TCR. 5. Alpha-interferon failed to augment the NK cell activity of lymphocytes from Sjogren patients, whereas gamma-interferon and IL-2 could enhance the NK cell activity. 6. Gamma-interferon, but not alpha-interferon could induce HLA-DR antigens on the cultured endothelial cells, enabling Leu- <3^+> T cells to adhere to the HLA-DR positive endothelial cells.
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Report
(1 results)
Research Products
(11 results)
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[Publications] Kajigaya,S., Kubota,K., Minato,N., Sudo,T., Hatake,K., Iizuka,M., Kobayashi,S., Saito,M., Kano,S. and Miura,Y.: "A murine cell line (2E10.4.13) produces five hemopoietic stimulators, and interlelukin-2 and interleukin-3." Cell Structure and Function. 10. 121-131 (1985)
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[Publications] Ikuta,K., Hattori,M., Wake,K., Kano,S., Honjo,T., Yodoi,J. and Minato,N.: "Expression and rearrangement of the alpha, beta, and gamma chain genes of the T cell receptor in cloned murine large granular lymphocyte lines. No correlation with the cytotoxic spectrum." Journal of Experimental Medicine. 164. 428-442 (1986)
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