| Project/Area Number |
59480239
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | The Jikei University School of Medicine |
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Principal Investigator |
AKATSUKA Jun-ichi Professor,Dept. of Pediatrics,The Jikei University School of Medicine, 医学部, 教授 (20056550)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIYAMA Hiroshi Assistant, the same as above, 医学部, 助手 (10168718)
ISHIDOYA Naoko Assistant, The same as above, 医学部, 助手 (80168244)
西野 仁美 慈恵医大, 医学部, 助手
FUJISAWA Kohji ASSISTANT, The same as above, 医学部, 助手 (10130197)
NISHINO Hitomi Assistant, The same as above
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1986: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1984: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | ITP / PAIgG / intravenous high-dose immunoglobulin therapy / Fc receptor / megakaryocyte / platelet target protein, IIbIIIa / PFC / ELISA-plaque法 |
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| Research Abstract |
In order to clarify the basic immunologic abnomalities of ITP in children, pathophysiologic significance of PAIgG were analysed from the view point of the difference of acute and chronic type. Then, further studies were directed towards developing of methods to enumerate PAIgG-secreting cells.The data obtained until now are as follows.
1. Determinations and clinical significance of PAIgG: 60 children with ITP,including 14 of acute and 46 of chronic type,were determined for PAIgG.PAIgG of children with ITP were remarkably higher than children with non immune thrombocytopenias. Changes of PAIgG after intravenous high-dose immunoglobulin therapy suggested that PAIgG were not always correlated to platelet counts and clearance of platelets loaded with PAIgG seemed to be influenced in relation to reticuloendotherial function and metabolism of immunoglobulin in peripheral circulation.
2. Fc receptor(FcR) of bone marrow megakaryocytes in ITP: Megakaryocytes were isolated from bone marrow aspirat
… More
es using density gradient centrifugation. Analysis of FcR positive megakaryocytes suggested the possibility to differenciate the mechanism of immunologic events involving this cell in which thrombocytopenias were due to immune complex- or autoimmune mediated phenomenon. Further studies are proceeding to investigate more situations in various kinds of disorders.
3. Detection of platelet membrane target antigens which bind anti-platelet antibodies in the sera of ITP: Immunoprecipitation and immunoblotting revealed no sera of 11 children with acute type and 6 sera of 29 children with chronic type which contained immunoglobulin to bind membraneus glycoprotein of platelets, IIbIIIa.
4. Developement of methods to enumerate platelet antibody-secreting lymphocytes in ITP: Numbers of methods to detect platelet antibody-secreting spleen cells were investigated using mice which were immunized with human platelets. PFC assay(Jerne) could not be applicable, because of difficulties of coating sheep red blood cells with membraneus protein of human platelets. On the contrary, ELIZA-plaque assay(Sedgwick,1983) were found to be useful method to detect spleen cells which were secreting antihuman platelet mouse antibody. This assay is now on study to detect PAIgG-secreting spleen cells and peripheral lymphocytes in children with ITP. Less
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