| Project/Area Number |
59480254
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Tokushima University (1985-1986)
Osaka University (1984) |
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Principal Investigator |
SHIMA Kenji Tokushima University School of Medicine, Professor, 医学部, 教授 (90028407)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Ichiro Tokushima University School of Medicine, Assistant, 医学部, 助手 (10178965)
HIROTA Meisei Tokushima University Hospital, Assistant, 医学部附属病院, 助手 (20173289)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1985: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1984: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | Jejunectomized rats / Liver perfusion / Gut hormones / cAMP / phosphopylase / Primary cultured hepatocyte / Enteroinsular axis |
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| Research Abstract |
Effects of the intestinal tract on carbohydrate metabolism was stueied in respect of its direct action on the metabolism in the liver and of the indirect effect through the pancreatic endocrine functions.
1. direct effects
(1) In the jejunectomized rats which had been nurished for a week by total parenteral nutrition the response of plasma glucose and IRI to an intraperitoneal glucose load was significantly higher than in the controls.
(2) Effects of various gut hormones on cAMP production, phosphorylase activity and glucose output were investigated in primary cultured hepatocytes. Glucagon, secretin and VIP augmented these three parameters, but others such as GRF, GIP and PHI did not stimulate all three.
2. A new candidate for incretin-like substance and a physiological significance of the enteroinsular axis. In response to an oral glucose load the plasma GLP-1 immunoreactivity level was increased in man and GLP-1 stimulated IRI release from the perfused rat pancreas. This might be the new incretin. It became clear that the enteroinsular axis acted on the pancreatic A cells as well on the basis of the fact that an increase in plasma glucagon level in response to arginin was augmented after an oral glucose load.
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