| Project/Area Number |
59480310
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
MINAMI Akio Hokkaido University Hospital Lecturer, 医学部, 助手 (20133738)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NATORI Takashi Hokkaido University, School of Medicine Instractor, 医学部, 助手 (00001892)
OGINO Toshihiko Hokkaido University Hospital Lecturer, 医学部附属病院, 講師 (60109436)
USUI Masamichi Sapporo Medical College Associate Prof., 助教授 (90002328)
TAKEICHI Noritoshi Hokkaido University, School of Medicine Associate Prof., 医学部, 助教授 (40002133)
|
|---|
| Project Period (FY) |
1984 – 1986
|
| Project Status |
Completed (Fiscal Year 1986)
|
| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1986: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1985: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1984: ¥3,200,000 (Direct Cost: ¥3,200,000)
|
|---|
| Keywords | Vascularized Joint Graft / Allogeneic Graft / Autologous Graft / Rat Major Histocompatibility Antigen / マイクロサージャリー |
|---|
| Research Abstract |
The experimental studies using rats were designed to investigate; (1)the effect of vascularity on autologous and allogeneic joint transplantations, (2)the influence of major histocompatibility antigen on survival and functioning of grafted joints.
Knee joints from 5 strains of the inbred rat were used in our study. The rats were classified into 2 groups. I:Vascularized autologous joint transplantation (from WKA to WKA rats) II:Vascularized allogeneic joint transplantation. Group II was further classified into 4 subgroups according to the difference of haplotypes (subregions) of rat major histocompatibility (RT1) antigens between the donor and recipient rat:1)RT1-A.B.D. barrier (from WKA to LEJ rats), 2)RT1-B.D. barrier (from TO to LEJ rats), 3)RT1-A. barrier (from BUF to LEJ rats), and 4)non-RT1 barrier (from WKA to W rats). Roentogenograms and 99m-Tc scintigrams were taken every 1 week after joint transplantation to investigate the time of bone healing and revascularization of transplanted joints. Histological studies were also performed.
Roentgenograms revealed that the bone healing began at 2.6 weeks post-operatively in Group I and Group II-4). The periosteal reaction of the transplanted joints wan not observed until 10 weeks post-operatively in Group II except in subgroup 4).
Scintigrams showed the immediate vascularity of transplanted joint in Group I and Group II-4). The vascularization of transplanted joint was observed until 2 weeks post-operatively in Group II-1), 2), and 3to 4 weeks in Group II-3). However, in Group II except in subgroup 4) bone, articular cartilage and surrounding muscle tissue became to be necrosis.
These studies suggest that the success of the vascularized allogeneic joint transplantation is dependent on the vascularity and low antigenicity of transplanted joint.
|
