| Project/Area Number |
59480324
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Akita University of Medicine |
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Principal Investigator |
KATO Tetsuro Department of Urology, 医学部, 助教授 (40004642)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Toshio Department of Pharmacy, 医学部, 助教授 (20108559)
UESUGI Shiro Department of Central Laboratory, 医学部, 教授 (20008246)
MORI Hisashi Department of Urology, 医学部, 助手 (10166366)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1985: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1984: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Nuclear Magnetic Resonance / Water Content / Relaxation Time / 膀胱腫瘍 |
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| Research Abstract |
1.Preservation and measuring condition of blood,urine and tissue samples: NMR relaxation times(T1 and T2) of tissues which were kept frozen by dry-ice remained almost stable within 24 hours after sampling. It was important to eliminate serum protein using trichloroacetic acid or a filter, because serum protein was an obstacle to judge a peak of lactate in NMR spectroscopic analysis of serum.
2.T1 and T2 through VX2 carcinoma growth: T1 values of tumor tissue gradually increased along with tumor growth, being greater than the control values which remained constant. T1 values of the non-tumorous bladder tissue were lower than those of the tumor throughout the middle stage of tumor growth(4 week), and then showed prominent increase at the end stage of tumor growth(5-6 weeks).
T2 value of the tumor tissue did not show any characteristic changes as compared with the controls throughout the progress of the tumor. T2 values of the non-tumorous bladder tissue also presented no peculiar change until the middle stage, but again showed a rapid increase as obseved in T1 monitoring, in the end stage.
T1 and T2 values in both the non-tumorous tisse and the control bladder tissue well correlated with the tissue water content throughout the tumor growth, while these on the tumor tissue represented no significant correlation with water content as a whole. However, the relaxation times in the tumor tissue well correlated with water content in the individual tumor stage,suggesting that relaxation times in tumor is influenced not only by water content but also by water-macrolmolecular interaction.
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