| Project/Area Number |
59480391
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SHIMOSATO Tunehiro HIROSHIMA UNIVERSITY SCHOOL OF DENTISTRY professor, 歯学部, 教授 (80028745)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Kohji HIROSHIMA UNIVERSITY SCHOOL OF DENTISTRY assistant, 歯学部(昭和61年度), 助手 (90179747)
OKUI Hiroshi HIROSHIMA UNIVERSITY UNIVERSITY DENTAL HOSPITAL assistant professor, 歯学部附属病院(昭和61年度), 講師 (20034225)
NAGAHATA Hikaru HIROSHIMA UNIVERSITY SCHOOL OF DENTISTRY assistant, 歯学部(昭和59.60年度), 助手 (90034256)
MITSUGI Masaharu HIROSHIMA UNIVERSITY SCHOOL OF DENTISTRY assistant, 歯学部(昭和59.60年度), 助手 (60127719)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1986: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1985: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1984: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | Cytology / Microphotometry Method / Radiotherapy / 化学療法 |
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| Research Abstract |
This study was done in order to establish more objective criteria of the treatment effects of radiotherapy or chemotherapy. For this aim, we applied a semiautoscanning microphotometry system connected to a computer with home made software. We characterized the nuclear atypia of cells for oral cancers during the course of the treatments. We measured the nuclear DNA by this system in order to judge the effect of radiotherapy on DNA. Then we measured an effective nuclear area, the total quantity of DNA and the average density of this quantity of DNA. In addition, we caliculated the average DS (Discriminant Score) and MGD (Malignant Generalized Distance). It is important to investigate how these parapeters changed with increasing doses of irradiation. These parameters peaked at 10Gy irradiation, and later showed a tendency to be similar to the quantity of normal cells. During the course of radiotherapy, we noted that the average DS was positive, and that it came comparatively close to a normal level. We also investigated how small the MGD was. As the fundamental examination of chemotherapy, we investigated various parameters of cell kinetics by using human salivary gland tumors in nude mice with this system and with [ <^3H> ]-thymidine autoradiography method. We investigated disturbed tissue or cells that were effected by antineoplastic (VCR) and their recovery process with these parameters. Our results showd that even if the tumor had a sesitivity to chemotherapy, it had a repropagative tendency after two or three weeks, and that the parameters in cell kinetics had the same tendency.
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