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Safety Assessment of Physical Energy Exposure to Ovarian Oocytes at Highly the Radiosensitive Stages of Oogenesis.

Research Project

Project/Area Number 59490003
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionAsahikawa Medical College

Principal Investigator

MIKAMO Kazuya  Asahikawa Medical College, Department of Biological Sciences, Professor, 医学部, 教授 (10002221)

Co-Investigator(Kenkyū-buntansha) TATENO Hiroyuki  Asahikawa Medical College, Department of Biological Sciences, Research Associate, 医学部, 教務職員 (80163492)
TANAKA Kunio  Asahikawa Medical College, Central Laboratory for Research and Education, Assoc., 医学部, 助教授 (20041840)
KAMIGUCHI Yujiroh  Asahikawa Medical College, Department of Biological Sciences, Assoc. Professor, 医学部, 助教授 (60091568)
Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1984: ¥6,300,000 (Direct Cost: ¥6,300,000)
KeywordsChinese hamstar oocyte / Neonate / X-rays / Ultrasound / Oocyte killing / Reproductive life span / Chromosome aberration / Dominant lethality / チャイニーズハムスター / 晩発効果 / 発生能
Research Abstract

We studied the effects of X-rays and ultrasound on ovarian oocytes of neonatal Chinese hamsters aged between 0 and 20 days with regard to (a) acute oocyte-killing and chromosome aberration in irradiated oocytes, (b) dominant lethality and congenital anomaly in the embryos developed from irradiated oocytes, and (c) reproductive life span of the irradiated females. The results are as follows :
(1) The immature oocytes dramatically changed their sensitivity to oocyte-killing effect of radiation, depending upon their meiotic stages of oogenesis. They were highly radioresistant at the pachytene stage, but became sharply sensitive during the phase between diplotene and early dictyate, suffering severe killing effect after 1 Gy of X-rays. However, the oocytes recovered radioresistance again at the following stage, i. e., the resting dictyate stage.
(2) Reproductive life span was markedly shortened in the females irradiated with 1 Gy at their neonatal stage (4 to 8 days of age) at which most ova … More rian occytes are in radiosensitive stages (diplotene to early dictyate). This indicates that the reduction of ovarian oocytes in number due to acute oocyte-killing is responsible for the shortening of reproductive life (premature menopause).
(3) There was no increase of chromosome aberrations in female pronuclei of one-cell embryos derived from the pachytene and resting dictyate oocytes which survived 1 Gy X-rays. No increase was also found in the incidences of pre- and postimplantation death and congenital malformations in the 18.5-day fetuses developed from survived oocytes. These findings suggest that pachytene and resting dictyate oocytes are highly capable of repairing DNA damage.
(4) Diplotene and early dictyate oocytes which survived low doses of X-rays (0.1-0.5 Gy) were free from late deleterious effects on genetic and developmental abilities.
(5) Exposure of ultrasound (3.25 W/cm^2) to radiosensitive oocytes (those in diplotene and early dictyate stages) induced neither acute oocyte-killing nor late effects due to genetic damage.
It has been generally believed that the late fetal and neonatal stages are relatively resistant to the teratogenic effects of mutagens such as radiation and chemicals. In this study, however, we demonstrated that premature menopause owing to oocyte-killing occurred after the irradiation during the radiosensitive periods. Considering that diplotene and early dictyate oocytes are also sensitive to some chemicals, the late fetal stages should be regarded as a critical period in determining fertility span of women. Fortunately, however, ultrasound seems to have a different nature from the radiations as far as the present study was concerned. Less

Report

(2 results)
  • 1990 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Tateno, H. & Mikamo, K.: "Neonatal oocyte development and selective oocyteーkilling by Xーrays in the Chinise hamster,<Cricetulus>___ー <griseus>___ー." International Journal of Radiation Biology. 45. 139-149 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 美甘 和哉: "妊娠前の雌に対する処置による発生障害" 先天異常. 24. 185 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tateno, H. & Mikamo, K.: "Reduction of female fertility span after Xーirradiation at neonatal stages, without teratogenic and cytogenetic effects on surviving oocytes." Japanese Journal of Human Genetics. 30. 140 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kamiguchi, Y. & Mikamo, K.: "Application of developmental biotechnology to chromosomal study of human spermatozoa." Japanese Journal of Human Genetics. 30. 85-87 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 立野 裕幸・美甘 和哉: "幼若卵子の放射線細胞死と生殖寿命の短縮" 「放射線の影響評価研究の現状と展望」 原子力安全研究協会編. 219-227 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 美甘 和哉・上口 勇次郎・船木 賢治・菅原 茂樹・小出 展久・立野 祐幸: "放射線の卵巣照射による卵子染色体異常と発生異常の誘発" 第15回放医研シンポジウム報文集 「放射線による遺伝損傷と リスクーその生物医学的アプロ-チ」 NIRSーMー54. 243-256 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tateno, H. & Mikamo, K.: "Absence of teratogenic and cytogenetic effects of Xーrays on pachytene oocytes in neonatal Chinese hamster." Journal of Radiation Research. 27. 44 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tateno, H. & Mikamo, K.: "Absence of late effects on survivar and developmental abilities of pachytene oocytes Xーirradiated during neonatal stages in the Chinese hamster." International Journal of Radiation Biology. 49. 121-130 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tateno, H., Mikamo, K., Kamiguchi, Y. & Tanaka, K.: "Acute and late effects on oocytes in Chinese hamsters Xーirradiated during neonatal stages." Zoological Science. 4. 1003 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tateno, H. & Mikamo, K.: "Correlation between Xーrayーinduced oocyteーkilling and fertility in the Chinese hamster." In “Radiation Research", Fielden,E.M. et al.(Eds) Taylor & Francis. 201 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kamiguchi, Y. & Mikamo, K.: "Spontaneous incidence of chromosome aberrations in mammalian zygotes." Zoological Science. 5. 1222 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tateno, H. & Mikamo, K.: "Effects of neonatal ovarian Xーirradiation in the Chinese hamster. I. Correlation between the age of irradiation and the fertility span." Journal of Radiation Research. 30. 185-190 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tateno, H. & Mikamo, K.: "Effects of neonatal ovarian Xーirradiation in the Chinese hamster. II.Absence of chromosomal and developmental damages in surviving oocytes irradiated at pachytene and resting dictyate stages." Journal Radiation Research. 30. 209-217 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 美甘 和哉: "初期発生における胚の淘汰" 日本医師会雑誌. 101. 221-226 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 美甘 和哉: "ヒトの生殖障害について:初期胚淘汰の生殖生物学的考察" 環境変異原研究. 11. 55-62 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 美甘 和哉: "ヒトでは初期胚淘汰がなぜ頻発するのか。" 医学のあゆみ. 150. 391-394 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 美甘 和哉: "ヒト胚淘汰の細胞遺伝学的考察" ペリネイタル ケア. 8. 932-938 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 上口 勇次郎: "生殖細胞などの培養と染色体分析 ー配偶子、受精卵、初期胚ー" 臨床病理 「最新の染色体分析 ー基礎と実際」 特集第80号. 299-309 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tateno. H., Kamiguchi, Y. & Mikamo, K.: "Meioticーstageーdependent radiosensitivity in ovarian oocytes of the mammal." Japanse Journal of Human Genetics. 36. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno and K. Mikamo: "Neonatal oocyte development and selective oocyte-killing by X-rays in the Chinese hamster, Cricetulus griseus." Intern. J. Radiat. Biol.45. 139-149 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Mikamo: "Deveropmental imparment caused by treatments to the female before conception." Cong. Anom.24. 185 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno and K. Mikamo: "Reduction of female fertility span after X-irradiation at neonatal stages, without teratogenic and cytogenetic effects on surviving oocytes." Jpn. J. Hum. Genet.30. 140 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Kamiguchi and K. Mikamo: "Application of developmental biotechnology to chromosomal study of human spermatozoa." Jpn. J. Human Genet.30 :. 85-87 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Mikamo, Y. Kamiguchi, K. Funaki, S. Sugawara, N. Koide and H. Tateno: "Induction of chromosome aberrations and developmental anomalies by radiation exposure to ovarian oocytes (in Japanese)." Proc. 15th NIRS Sympo., Nakai, S. et al. (Ed.), National Institute of Radiological Sciences, Chiba,. 243-256 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno and K. Mikamo: "Oocyte-killing and shortening of reproductive life span induced by X-irradiation to immature ovarian oocytes." "The Present Situation and Future Prospect on the Assessment Study of Radiation Effects" (in Japanese), Nuclear Safety Research Association (Ed.), Tokyo,. 219-227 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno and K. Mikamo :"Absence of teratogenic and cytogenetic effects of X-rays on pachytene oocytes in neonatal Chinese hamster." J. Rabit. Res. 27 :. 44 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno and K. Mikamo: "Absence of late effects on survival and developmental abilities of pachytene oocytes X-irradiated during neonatal stages in the Chinese hamster" Int j. Radiat. Biol.49 :. 121-130 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno, K. Mikamo, Y. Kamiguchi, and K. Tanaka :"Acute and late effects on oocytes in Chinese hamsters X-irradiated during neonatal stages." Zool. Sci.4 :. 1003 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno and K. Mikamo :"Correlation between X-ray-induced oocyte-killing and fertility in the Chinese hamster." "Radiation Research", Feilden, E. M. et al. (Ed.), Taylor and Francis, London,. 201 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Kamiguchi and K. Mikamo: "Spontaneous incidence of chromosome aberrations in mammalian zygotes." Zool. Sci.5 :. 1222 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno and K. Mikamo :"Effects of neonatal ovarian X-irradiation in the Chinese hamster. I. Correlation between the age of irradiation and the fertility span." J. Radiat. Res.30 :. 185-190 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno and K. Mikamo :"Effects of neonatal ovarian X-irradiation in the Chinese hamster. II. Absence of choromosomal and developmental damages in surviving oocytes irradiated at pachytene and resting dictyate stages." J. Radiat. Res.30 :. 209-217 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Mikamo :"Embryonic loss during early development (in Japanese)." J. Jpn. Med. Assoc.101 :. 221-226 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Mikamo :"Reproductive failure in man : Early embryonic loss viewed from the angle of reproductive biology (in Japanese)." Environ Mutagen Res. Com.11 :. 55-62 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Mikamo :"Reasoning for the early embryonic loss occurring frequently in human (in Japanese)." J. Crin. Exp. Med. (Igaku no Ayumi). 150 :. 391-394 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Mikamo :"Cytogenetics of the embryonic loss in human (in Japanese)." Perinatal Care. 8 :. 932-938 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Kamiguchi :"Methods for culture and chromosome analysis of the gamete (in Japanese)." Jpn. J. Clin. Pathol.80 :. 299-309 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Tateno, Y. Kamiguchi, and K. Mikamo: "Meiotic-stage-dependent radiosensitivity in ovarian oocytes of the mammal." Jpn. J. Genet.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Tateno;K.Mikamo: Int.J.Radiat.Biol.45. 139-149 (1984)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Tateno;K.Mikamo: Jpn.J.Hum.Genet.30. 140 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Tateno;K.Mikamo: Int.J.Rabiat.Biol.49. 121-130 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Tateno;K.Mikamo: J.Radiat.Res.27. 44 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 立野裕幸,美甘和哉: "放射線の影響評価研究の現状と展望" (財)原子力安全研究協会, 312 (1985)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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