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Analysis of mechanisms of controlling <Ca^(2+)> -channels in myocardium transplanted into the renal capsular space of the rat

Research Project

Project/Area Number 59570086
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKumamoto University

Principal Investigator

NISHI Katsuhide  Kumamoto University Medical School, 医学部, 教授 (00040220)

Co-Investigator(Kenkyū-buntansha) KAWAMURA Tomomi  Kumamoto University Medical School, 医学部, 助手 (00153015)
KADOWAKI Yoshihiro  Kumamoto University Medical School, 医学部, 助手 (10145310)
Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1985: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1984: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsIon-selective microelectrode / intracellular Na activity / transplanted myocardium / 細胞内Na活性 / 細胞内K活性 / イオン選択性電極
Research Abstract

1. We measured changes in membrane potential (MP), extra-and intracelllar potassium and intracellular sodium ion activities ( <a^o_k> , <a^i_k> and a <N^i_a> ) in papillary muscle of the guinea-pig heart induced by repetitive stimulation at various frequencies and periods in vitro, using double barrelled ion-selective microelecrodes. Stimulation (2-4Hz) depolarized the cells, and termination of stimulation induced hyperpolarization. <a^i_k> and a <n^i_a> (stimulated at 0.1 Hz) were 92.3 <-!+> 4.6 mM, and 7.8 <-!+> 2.0mM (mean <-!+> SD), respectively. Prolongation of the stimulation period (0.5-2 min) increased a <N^i_a> timedependently, but there was no further increase by stimulation for longer than 3 min. The increase in a <N^i_a> was dependent on stimulus-frequency. Afer termination of the stimulation, a <N^i_a> declined exponentially. <a^i_k> slightly decreased by the stimulation (3-4Hz). <a^o_k> increased during the stimulation period, and decreased below the initial level after t … More ermination of the stimulation. Results suggests that in guinea-pig papillary muscle, the contribution of an electrogenic component of Na-K pump to the hyperpolarization after stimulation would be small, since the hyperpolarization could be explained mostly by depletion of <a^o_k> induced by Na-K pump activity.
2. We have developed a new model for electrophysiological studies on ischemic myocardium in situ. Pieces of ventricullar tissue(lxlmm) excised from the neonatal rat heart were transplanted into the renal capsular space of the adult rat of the pure strain F344-NSlc. Electrophysiological properies of the transplanted cells during normal circulation and during ischemia induced by ligation of the renal artery were examined in situ 7-10 days later, when the tissues were fully vascularized, by means of conventional microelectrode techniques. Transmembrane potentials varied in individual cells, ranging from -60 to -90mV. The amplitude, duration, and configuration of spontaneously occuring action potentials recorded from the transplanted cells were similar to those obtained in neonatal and adult rat myocardium. Less

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 西勝英: 心臓. 17. 467-476 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y.Nishizono: ADVANCES IN MYOCARDIOLOGY. 6. 383-393 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] TATSUO KANO: American Journal of Physiology. 251. H324-H330 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] K. Nishi: "A model for ischemic myocardium-electrophysiological properties of myocardium transplanted tnto the renal capsular space" Shinzo. 17. 467-476 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y. Nishizono: "A Model for Cardiac Ischemia Cardiac cells Trasplanted into the Renal Capsular Space of the Rat and Their Electrophysiological Properties" ADVANCES IN MYOCARDIOLOGY. 6. 383-393 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] TATSUO KANO: "External pH dependency of delayed afterepolarization in rabbit myocardium" American Jounal of Physiology. 251. H324-H330 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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