Studies on Clostridium difficile enterotoxin

Research Project

Project/Area Number	59570185
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	細菌学
Research Institution	Gifu University School of Medicine
Principal Investigator	UENO Kazue Gifu University School of Medicine, 医学部, 教授 (20021363)
Co-Investigator(Kenkyū-buntansha)	MUTOH Yoshinori Gifu University School of Medicine, 医学部, 助手 (80190859) KATOH Naoki Gifu University School of Medicine, 医学部, 助手 (00108078) WATANABE Kunitomo Gifu University School of Medicine, 医学部, 講師 (70108067)
Project Period (FY)	1984 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥1,600,000 (Direct Cost: ¥1,600,000) Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000) Fiscal Year 1985: ¥400,000 (Direct Cost: ¥400,000) Fiscal Year 1984: ¥800,000 (Direct Cost: ¥800,000)
Keywords	C.difficile / Enterotoxin / Antibiotic-associated diarrhea / 小腸粘膜の変化;病院内感染 / C.perfringens typeA
Research Abstract	Measurement of C.difficile and their toxin in fecal samples of antibiotic associated diarrhea; The results showed that the detection of the C.difficile and C.difficile enterotoxin were extremely correlated. 4 cases out of 11 were positive for both C.difficile and their enterotoxin, and 7 cases were found to be negative for both C.difficile and their enterotoxin. Measurement of C.difficile enterotoxin showed that fecal samples from healthy subjects contained 0 - 255 ng/ml toxin, on the other hand, that of the patients with antibiotic-associated diarrhea ranged between 1500 - 6500 ng/ml. We suggested that C.difficile enterotoxin may be one of the main causative toxin for the antibiotic-associated diarrhea or pseudomembranous colitis. Changes of intestinal microflora on the antibiotic-associated diarrhea due to C.difficile; The results showed that Enterococcus faecalis in intestinal contents may be important for the over growth of C.difficile in the intestine who had antibiotics.