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Studies on the Molecular Structure and Function of Human Monoclonal Cryoimmunoglobulins.

Research Project

Project/Area Number 59570223
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionSchool of Medicine, University of Occupational and Environmental Health, Japan

Principal Investigator

NAKAMURA Hiroshi  Dept. of Immunol., Schl of Med., Univ. of OEH., Professor, 医学部, 教授 (60012720)

Co-Investigator(Kenkyū-buntansha) SUGIURA Tsutomu  Dept. of Immunol., Schl. of Med., Univ. of OEH., Assistant, 医学部, 助手 (40131924)
NISHIMURA Yoshihisa  Dept. of Immunol., Schl of Med., Univ. of OEH., Associate Professor, 医学部, 助教授 (90092288)
YAMASHITA Uki  Dept. of Immunol., Schl. of Med., Univ. of OEH., Associate Professor, 医学部, 助教授 (00028680)
Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1984: ¥800,000 (Direct Cost: ¥800,000)
KeywordsHuman monoclonal cryoglobulins / IgG3 and IgM / Cryoprecipitability / IgG3 / IgM
Research Abstract

Cryoglobulin are variety of immunoglobulins which form precipitates or gels at temperatures below 37゜ C, and are usually found in plasma of patients having chronic infectious diseases and various lymphoproliferative disorders. The clinical features are recognized by vasoconstriction of skin capillaries triggered by deposition of immunoglobulin and also by increasing viscosity of serum caused by aggregation of immunoglobulin at low temperatures. The reason for the decreased solubility of monoclonal type of cryoglobulins is not yet understood, but it appears to reflect the structure of a small and restricted part of the molecules, because of no significant difference in the primary structures between cryoglobulins and myeloma proteins belonged to the same class or subclass. In this study, the immunochemical and physico-chemical characteristics of cryoglobulins were presented by comparison with that of the myeloma proteins as control proteins, and the results were summarized as follows; 1 … More . the specific association between homologous cryglobulin molecules, that is, no cryo-coprecipitation between cryoglobulins belonged to the same class or subclass from different patients. 2. the molecular rigidity of cryoglobulin in solution characterized by gelfiltration and viscosity measurements, and by fluorescence polarization measurements using DNS-conjugated proteins. 3. the involvement of hydrophobic amino acid residues in cryoprecipitation. 4. the characteristic CD spectra for cryoglobulins associated with the temperature-dependency. In addition, the results were obtained that the cryoprecipitate formation was susceptively inhibited by niacinamide at concentrations higher than 0.2M, and also the similar effect on antibody-antigen precipitate formation was observed by the reagent and the derivertives, suggesting that the inter-molecular interaction between hydrophobic residues might be involved in the association of cryoglobulins and the development of antibody-antigen complexes. Less

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yoshihisa,NISHIMURA;Hiroshi,NAKAMURA: Journal of Biochemistry. 95. 255-265 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroshi NAKAMURA;Yoshihisa,NISHIMURA: Cellular,Molecular and Genetic Approaches to Immunodiagnosis and Immunotherapy. 31-35 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroko,SAKAI;Hiroshi,NAKAMURA: Journal of University of Occupational and Environmental Health. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Junko,AKIYOSHI;Hiroshi,NAKAMURA: Journal of University of Occupational and Environmental Health. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroshi,NAKAMURA;Mariko,ETOH: Journal of Biochemistry. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 中村弘 著村松繁,増田徹,桂義元 編: "実験生物学講座第14巻 免疫生物学 4.抗原と抗体の定性・定量分析法" 丸善株式会社, 71-83 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 中村弘,杉浦勉: "続生化学実験講座第5巻 日本生化学会編 免疫生化学研究法 1.2 2.4" 東京化学同人,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yoshihisa, NISHIMURA and Hiroshi,NAKAMURA: "Human Monoclonal Cryoimmunoglobulins. <I> Molecular Properties of IgGk(Jir Protein) and the Cryo-Coprecipitability of Its Molecular Fragments by Papain" Journal of Biochemistry. 95. 255-265 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroko, SAKAI and Hiroshi NAKAMURA: "Human Monoclonal Cryoimmunoglobulins. <II> Analysis for the Carbohydrate Moiety of Cryoimmunoglobulins" Journal of University of Occupational and Environmental Health. in preparatin (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Junko, AKIYOSHI and Hiroshi NAKAMURA: "Human Monoclonal Cryoimmunoglobulins. <III> Analysis of the Hydro-dynamic Properties of DNS-conjugated Proteins by Fluorescence Polarization Measurement" Journal of University of Occupational and Environmental Health. in preparation (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroshi, NAKAMURA and Mariko, ETOH: "Effects of Niacinamide on Inter-molecular Association of Immunoglobulin Molecules." Journal of Biochemistry. in preparation (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroshi, NAKAMURA and Yoshihisa NISHIMURA: University of Tokyo Press. Dynamic Aspects on Inter-molecular Association of Immunoglobulin Molecules. in Cellular, Molecular and Genetic Approaches to Immunodiagnosis and Immunotherapy., 31-35 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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