| Project/Area Number |
59570431
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Kurume University |
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Principal Investigator |
OGURA Ryohei Kurume University School of Medicine , Professor, 医学部, 教授 (10080566)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Masayasu Kurume University School of Medicine , Research Associate, 医学部, 助手 (40154504)
HIDAKA Toshihiro Kurume University School of Medicine , Associate Professor, 医学部, 助教授 (10113234)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1984: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | B-16 melanoma cell / ultraviolet exposure / <Ca^(2+)> / phospholipase / arachidonate / calmodulin / membrane fluidity / spin label / 過酸化脂質 |
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| Research Abstract |
To examine the physiological responses of the cell membrane following UV exposure, B-16 melanoma cells in culture were prelabelled with [ <^3H> ]-arachidonate and exposed to UV radiation. Immediately after irradiation the cells released labelled arachidonate. The UV-stimulated arachidonate release was inhibited by mepacrine and calmodulin inhibitor W-7. The release of arachidonate decreased significantly at lower <Ca^(2+)> concentrations (5 <micro> M), but did not increase at higher <Ca^(2+)> (2 mM). In addition, phospholipase <A_2> and C activiteis were identified. By the method of electron spin resonance with spin label technique, membrane fluidity decreased immediately being accompanied with lipid peroxidation after UV exposure, and then increased 6 hrs after UV expousre.
From these results, it was demonstrated that accumulation of lipid peroxide results in the decrease of membrane fluidity. These results suggested that <Ca^(2+)> dependent calmodulin-dependent phospholipases were involved in UV stimulated arachidonate release from the cells.
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