| Project/Area Number |
59570537
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Tokyo University |
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Principal Investigator |
YAMAKAWA Mitsuru Tokyo University,Faculty of Medicine, 医学部, 講師 (30167697)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Yoshio Tokyo University,Faculty of Medicine, 医学部(分), 前教授 (30010507)
OOHARA Takeshi Tokyo University,Faculty of Medicine, 医学部(分), 教授 (20010217)
FURUYA Kiyokazu Tokyo University,Faculty of Medicine, 医学部(分), 助教授 (30010523)
MAEDA Jun Tokyo University,Faculty of Medicine, 医学部(分), 助手 (30165641)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1984: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Carnitine / Fat Emulsion / Sepsis / Diabetese / Hepatic / dysfunction / Free Carnitine / Acylcarnitine |
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| Research Abstract |
Energy metabolism of fat emulsion has been studied in rats with various conditions including postoperative catabolism,chronic and acute hepatic dysfunction,diabetes and sepsis. The results indicate that fat emulsion is rapidly eliminated from the blood stream and metabolized readily even in such conditions. Based on these findings,we have employed fat emulsion clinicaly as energy source.
To clarify metabolic sequence of intravenously adiministered fat emulsion in these conditions,the tissue concentration of carnitine,which plays an important role in oxidation of long chain fatty acid,is studied by an enzymatic methods in the rats. In septic rats,it was demonstrated that the levels of free carnitine decreased and that this decrease was based upon a decrease of synthesis. When carnitine was administered together with fat emulsion the energy metabolism returned to approximatery normal level. Acute diabetic condition was induced by intravenous adiministration of 65mg/kg streptozotocin. In 4 days after the procedure,free carnitine was markedly increased in the liver and short chain acylcarnitine was increased in the muscles as well as in the kidney. These results indicate increased production and distribution of carnitine in the diabetic condition.
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