| Project/Area Number |
59570538
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Niigata University |
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Principal Investigator |
YOSHIKAWA Keiji Associate Professor, Niigata University, 医学部, 助手 (60143765)
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| Co-Investigator(Kenkyū-buntansha) |
KOYAMA Shin Associate Professor, Niigata University, 医学部, 助教授 (90018317)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1984: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | endogenous glucose production rate / 6- <^3H> -glucose / primed-constant infusion method / total pancreatectomy / endotoxemia / hepatectomy / <^(14)C> -Carbonate method / アルブミン合成 |
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| Research Abstract |
1. Studies on hepatic glucose production under the various surgical conditions. Glucose kinetics were determined by the Primed-Constant Infusion Method using 6- <^3H> -glucose.
1) Total pancreatectomy: Male mongrel dogs underwent total pancreatectomy with total gastrectomy were divided into two groups according to the difference of postoperative management. Group A received 0.2 U/Kg/day of insulin. Group B was given no insulin. Hepatic glucose production rate (Ra) and blood sugar levels (BS) were remarkably elevated in group B. Ra and BS in dogs of group A were controlled at the same levels as sham operation group. Hyperaminoacidemia mainly caused by the elevation of the glucogenic amino acids levels was observed in both groups. These amino acid derangements were normalized by the exogenous glucagon administration accompanied by the changes of Ra and BS.
2) 70% hepatectomy: Glucose production rate per unit weight of remnant liver was remarkably increased in order to meet the peripheral glucose utilization.
3) Endotoxemia: Both the hepatic glucose production rate (Ra) and the peripheral glucose utilization rate (Rd) were remarkably enhanced in dogs received E. coli endotoxin (LD 100). Rd was greater than Ra, and hypoglycemia ensued. Enhanced Rd was attributed to increased glucose oxidation.
2. Studies on the determination of in vivo hepatic albumin synthesis using <^(14)C> -carbonate method. The technical details of this method was clarified. Mongrel dogs after the 24hr fasting showed wide variation of the synthesis rate among individuals. Further studies on albumin synthesis under various surgical conditions will be carried out.
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