• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Prostaglandins Metabolism in Uterine Placental Fetal System

Research Project

Project/Area Number 59570714
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionMIYAZAKI MEDICAL COLLEGE

Principal Investigator

MORI Norimasa  Miyazaki Medical College, Professor, 医学部, 教授 (80040559)

Co-Investigator(Kenkyū-buntansha) KOIKE Hiroyuki  Miyazaki Medical College, Instructor, 医学部附属病院, 講師 (40128377)
YAMAGUCHI Masatoshi  Miyazaki Medical College, Research associate, 医学部附属病院, 助手 (90174630)
LEE Keisan  Miyazaki Medical College, Research associate, 医学部附属病院, 助手 (30167051)
KAWANO Michihisa  Miyazaki Medical College, Research associate, 医学部, 助手 (00136813)
Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1984: ¥800,000 (Direct Cost: ¥800,000)
KeywordsUterine placental fetal system / Prostaglandins / Prostaglandin dehydrogenase / Leukotrienes / Amniotic fluid embolism / Leukotriene阻害剤 / 羊水塞栓症
Research Abstract

Placental prostaglandin (PG) dehydrogenase (PPGDH) and PG producing activity in the uterine placental fetal system were studied.
1. Specific activity of PGDH purified by ammonium sulfate precipitation,DEAE-cellulose (DE-52), Sephadex G-100 gel filtration, NAD-affinity chromatography (AGNAD type 1), and the gel filtration high performance liquid chromatography (GFHPLC) was increased from 1.5x <10^(-2)> unit/mg of the initial supernatant to 1272x <10^(-2)> after GFHPLC. PGDH showed a single band on SDS-polyacrylamide gel electrophoresis and the molecular weight determined by GFHPLC and the electrophoresis was approximately 50,000 daltons. After 4 months of storage, PGDH in a buffer containing 50% glycerol at -20゜C retained 80% of its activity. Michaelis constant for PGE was 0.4 <mu> M at pH 7.0 with NAD as a cofactor.
2. Indomethacin and Puromycin caused IUGR of rat and suppressed the activity of PGDH of rat placenta. Severity of the rat IUGR and the suppesion of PGDH activity by indometha … More cin were not parallel. Therefore, the mechanism of indomethacin induced IUGR seemed to be independent from the suppresion of placental PGDH.
3. PG <I_2> producing activity of rat pregnant uterus was measured. The activity at placental site and placental opposite site was increased as pregnancy progressed. 4. The in vitro effects of obstetric drugs on PGDH activity were studied. Enzyme activity was inhibited by indomethacin, methylergometrine,Solcoseryl, conjugated estrogen, progesterone,and was activated only by isoxsuprine-HCl. Methylergometrine maleate and isoxsuprine-HCl, which have opposite functions on uterine muscle, also exerted contrary effects on enzyme activity.
5. Extracts of experimental amniotic fluid embolism from rabbit lungs showed the same biological activities as leukotrienes and a peak on HPLC at the same retention time as LT <D_4> . Acute death was prevented by administration of 5-lipoxygenase inhibitor. These results suggest the possibility that leukotrienes contribute to the clinical and pathophysiological features of amniotic fluid embolism. Less

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 鶴田憲一,他: 九州実験動物研究会会報. 2. 35-41 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tetsuji Tanaka et al: Biochemical and Biophysical Research Communications. 135. 1058-1063 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tetsuji Tanaka et al: Prostaglandins,Leukotrienes and Medicine. 23. 267-275 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao Azegami et al: American Journal of Obstetrics and Gynecology. 155. 1119-1123 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masatoshi Yamaguchi et al: Prostaglandins,Leukotrienes and Medicine.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kimihiro Nagai et al: Prostaglandins,Leukotrienes and Medicine.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tetuji Tanaka et al: "Labile oligometric structure of human placental 15-hydroxyprostaglandin dehydrogenase" Biochem.Biophy.Res.Commu.135. 1058-1063 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Tetuji Tanaka et al: "Specific activity staining for prostaglandin metabolizing enzymes on polyacrylamide" Prostaglandins,Leukotrienes and Medicine. 23. 267-275 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao Azegami et al: "Amniotic fluid embolism and Leukotriene" Amer.J.Obstet.Gynecol.155. 1119-1123 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kimihiro Nagai et al: "Purification of an NAD -dependent 15-hydroxyprostaglandin dehydrogenase from the human placenta" Prostaglandins,Leukotrienes and Medicine.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] masatoshi Yamaguchi: "Prostaglandin production by human myometrium, uterine cervix and leiomyoma" Prostaglandins,Leukotrienes and Medicine.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi