| Project/Area Number |
59570714
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | MIYAZAKI MEDICAL COLLEGE |
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Principal Investigator |
MORI Norimasa Miyazaki Medical College, Professor, 医学部, 教授 (80040559)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Hiroyuki Miyazaki Medical College, Instructor, 医学部附属病院, 講師 (40128377)
YAMAGUCHI Masatoshi Miyazaki Medical College, Research associate, 医学部附属病院, 助手 (90174630)
LEE Keisan Miyazaki Medical College, Research associate, 医学部附属病院, 助手 (30167051)
KAWANO Michihisa Miyazaki Medical College, Research associate, 医学部, 助手 (00136813)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1984: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Uterine placental fetal system / Prostaglandins / Prostaglandin dehydrogenase / Leukotrienes / Amniotic fluid embolism / Leukotriene阻害剤 / 羊水塞栓症 |
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| Research Abstract |
Placental prostaglandin (PG) dehydrogenase (PPGDH) and PG producing activity in the uterine placental fetal system were studied.
1. Specific activity of PGDH purified by ammonium sulfate precipitation,DEAE-cellulose (DE-52), Sephadex G-100 gel filtration, NAD-affinity chromatography (AGNAD type 1), and the gel filtration high performance liquid chromatography (GFHPLC) was increased from 1.5x <10^(-2)> unit/mg of the initial supernatant to 1272x <10^(-2)> after GFHPLC. PGDH showed a single band on SDS-polyacrylamide gel electrophoresis and the molecular weight determined by GFHPLC and the electrophoresis was approximately 50,000 daltons. After 4 months of storage, PGDH in a buffer containing 50% glycerol at -20゜C retained 80% of its activity. Michaelis constant for PGE was 0.4 <mu> M at pH 7.0 with NAD as a cofactor.
2. Indomethacin and Puromycin caused IUGR of rat and suppressed the activity of PGDH of rat placenta. Severity of the rat IUGR and the suppesion of PGDH activity by indometha
… More
cin were not parallel. Therefore, the mechanism of indomethacin induced IUGR seemed to be independent from the suppresion of placental PGDH.
3. PG <I_2> producing activity of rat pregnant uterus was measured. The activity at placental site and placental opposite site was increased as pregnancy progressed. 4. The in vitro effects of obstetric drugs on PGDH activity were studied. Enzyme activity was inhibited by indomethacin, methylergometrine,Solcoseryl, conjugated estrogen, progesterone,and was activated only by isoxsuprine-HCl. Methylergometrine maleate and isoxsuprine-HCl, which have opposite functions on uterine muscle, also exerted contrary effects on enzyme activity.
5. Extracts of experimental amniotic fluid embolism from rabbit lungs showed the same biological activities as leukotrienes and a peak on HPLC at the same retention time as LT <D_4> . Acute death was prevented by administration of 5-lipoxygenase inhibitor. These results suggest the possibility that leukotrienes contribute to the clinical and pathophysiological features of amniotic fluid embolism. Less
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