The receptor of chemical mediator on inflammation cell membrane in tissue with periodontitis.

Research Project

Project/Area Number	59570812
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Conservative dentistry
Research Institution	Meikai University
Principal Investigator	KUSUNOKI Kimihito Josai Dental University, Dental department, associate professor, 歯学部, 助教授 (50120431)
Co-Investigator(Kenkyū-buntansha)	SHIMOJIMA Takahiro Josai Dental University, Dental department, assistant, 歯学部, 助手 (60146230) FUJIHASHI Hiroshi Josai Dental University, Dental department, assistant, 歯学部, 助手 (50129128)
Project Period (FY)	1984 – 1985
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥1,700,000 (Direct Cost: ¥1,700,000) Fiscal Year 1985: ¥200,000 (Direct Cost: ¥200,000) Fiscal Year 1984: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywords	Histamine / Neutrophil / Histamine receptor / Bacteroides gingivalis / Neutrophil chemotaxis / 好中球走化能
Research Abstract	The present study was carried out to make clear the influence of histamine and Bacteroides gingivalis sonicated supernatant to the function of neutrophil, especialy in chemotaxis. First, in Macaca monkey, the influence of histamine to histamine receptor on neutrophil cell membrane in keeping with progress of the inflammation in experimental gingivitis were investigated by histamine coated SRBC rosette formation assay. Secandly, histamine coated Sepharose rosette formation assay was carried out to demonstration of existence of <H_2> receptor on neutrophil cell membrane in peripheral blood from clinically healthy individuals, and to examination of the influence of histamine to the function of neutrophil chemotaxis to FMLP by modified Boyden chamber method was done. Thirdly, with above assay, the influence of Bacteroides gingivalis sonicate supematant to resette formation and to neutrophil chemotaxis through histamine receptor on neutrphil cell membrane were examined. The result were as follows. 1. The change in number of histamine receptor on neutrophil cell membrane in keeping with progress of the inflammation in experimental gingivitis were observed. 2. The existence of <H_2> receptor on neutrophil cell membrane were confirmed and observed that histamine ( <10^(-5)> Mol) inhibited the neutrophil chemotaxis through <H_2> receptor. 3. The neutrophil chemotaxis and rosette formation between histamine coated Sepharose and neutrophil were enhanced by Bacteroides gingivalis sonicated supernatant. But, it's effect was inhibited by histamine ( <10^(-5)> Mol) and <H_2> receptor antagonist (cimetidine).