| Project/Area Number |
59570874
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | Showa University |
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Principal Investigator |
YAMASAKI Kenichi Showa University,School of Dentistry,Assistant Protessor, 歯学部, 助教授 (10124702)
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| Co-Investigator(Kenkyū-buntansha) |
SAITOH Shigeru Showa University,School of Dentistry Phd Student, 歯学部, 大学院生 (20195986)
SHIBATA Yasunori Showa University,School of Dentistry,Assistant, 歯学部, 助手 (50138400)
HIRAIDE Takatoshi Showa University,School of Dentistry, Lecturer, 歯学部, 講師 (80129866)
FUKAHARA Tatsuo Showa University,School of Dentistry, Protessor, 歯学部, 教授 (50013834)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,550,000 (Direct Cost: ¥1,550,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1984: ¥850,000 (Direct Cost: ¥850,000)
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| Keywords | orthodontic tooth movement / bone resorptive activity / periodontal tissue organ culture / chemical mediator / Prostaglandin E / プロスタサイクリン / トロンボキサンA_2 / 【^(45)Ca】 / 凍結割断 / ケミカルメディエーター / Waldo法 |
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| Research Abstract |
The aim of the present study are to transfer the periodontal tissues received experimental tooth movement into organ culture(in vitro) system,and to measure the bone resporptive activity of them. Using this system will clear up mechanizms of many kind of drugs and hormones on the rate of orthodontic tooth movement. 1. Establishment of experimental tooth movement; Rat incisors were fitted lateral expanding helical torsion loops(30g) During 1 day to 7 days. This experimepnt induced the appearance of osteoclasts and bone resorption of buccal site of alveolar bone.
2.Establishment of bone culture system for measurement of bone resorptive activity ;Neo-natal mice(1-day-old) were labelled^<45>Cacl_2. 4 days later,calvariae were dissected out and transferred to BGJB medium supplemented with 5% horse serum and antibiotics. Addition of PTH,PGE_2,PGl_2 analog(Op41483) and TXA_2 analog(STA_2) to this culture medium induced bone resorption (the rate of ^<45>Ca release from calvariae). So,this system will be able to measure the bone resorptive activity in vitro.
3. Examination of organ culture of periodontal tissues received experimental tooth movement; The extracted periodontal tissues showed life-activity within 24 hours in the above organ culture system.
4.The effect of periodontal tissues received experimental tooth movement on bone resorption in vitro; In spite of difficulty of preparation of experiment in vitro system,the difference of bone resorptive activity between control and treated periodontal tissues can be detectable.
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