| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1984: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
Saikosaponin a (sa) and c (sc) were treated with rat or artificial gastric juice to produce saikosaponin <b_1> ( <sb_1> ) and h (sh), possessing heteroannular diene at C-11, 13(18), and saikosaponin g (sg) and i (si), possessing homoannular diene at C-9(11), 12. On the other hand, saikosaponin d (sd) gave only saikosaponin <b_2> ( <sb_2> ), possessing heteroannulardiene, by the same treatment. Then,the incubation of sa, <sb_1> , sg, sd and <sb_2> with mouse intestinal flora gave the partial hydrolysis compounds, prosaikogenin F, A, H, G and D (PSF, PSA, PSH, PSG and PSD) and saikogenin F, A, H, G and D (SF, SA, SH, SG and SD). Although, sc, sh and si gave saikogenin E, C and B (SE, SC and SB), by the treatment with intestinal flora, prosaikogenins were not detected due to their complexity. Therefore, the new cleavage reaction of the glycosidic bond of saponin in alkoholic metal solution containing a trace of water. This reaction never cleaved the ether ring of sc and and gave 9 kinds o
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f prosaikogenins, prosaikogenin <E_1> (PS <E_1> :glc-glc-SE), <E_2> (PS <E_2> :rham-glc-SE) and <E_3> (PS <E_3> :glc-SE), prosaikogenin <C_1> (PS <C_1> :glc-glc-SE), <C_2> (PS <C_2> :rham-glc-SE) and <C_3> (PS <C_3> :glc-SE), and prosaikogenin <B_1> (PS <B_1> :glc-glc-SE), <B_2> (PS <B_2> :rham-glc-SE) and <B_3> (PS <B_3> :glc-SE). The 30 kinds of saikosaponin derivatives those were derived from the oral administration of sa, sc and sd in the alimentary tract were absorbed into the blood stream. Then, the effects of these derivatives on the corticosterone (endogenous glucocorticoid) secretion was investigated. Among sa and sd metabolites, sa and sd showed the strong increasing activity. However, sc was inactive and PS <E_1> and PS <E_2> stimulated the corticosterone secretion among sc derivatives. These results show that the stimulation of pituitary-adrenocortical axis function by saikosaponin metabolites could be due to the differencese of the molar polarity which depends uppon the existence of ether ring and the diffent glycosidic chain. Less
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