| Project/Area Number |
59840020
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
HIRAMOTO Yukio Faculty of Science, Tokyo Institute of Technology, 理学部, 教授 (50011440)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIJIMA Sumio Department of Cell Biology, National Institute for basic Biology, 非常勤講師 (70193315)
HAYAKAWA Tsuyoshi Hamamatsu Photonics K.K., K.K., 研究部主任
YOSHIMOTO Yasuaki Department of Cell Biology, National Institute for basic Biology, 助手 (30124225)
HAMAGUCHI Yukihisa Faculty of Science, Tokyo Instityte of Technology, 理学部, 助教授 (70016161)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥31,900,000 (Direct Cost: ¥31,900,000)
Fiscal Year 1986: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1985: ¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1984: ¥21,700,000 (Direct Cost: ¥21,700,000)
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| Keywords | Intracellular <Ca^(2+)> / aequorin / high-sensitive video-microscopy / fertilization / cleavage / amoeba / シラタマモ / 分裂周期 / 顕微鏡 / 高感度テレビ / 細胞内カルシウム |
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| Research Abstract |
A video-microscope system was developed, in order to visualize microscopic objects under an extremely-low intensity light. Using this system, intracellular release of calcium ions at fertilization and artifical activation was recorded in eggs of sea urchin, starfish, medaka, frog and golden hamster, which had been injected with aequorin, a photoprotein sensitive to free calcium ions. In these eggs, the increase in intracellular calcium ion concentration was started at the site of sperm entry or the site of artificial activation and it propagated over the cell cortex toward the antipod. This calcium wave preceded the wave of the breakdown of cortical alveoli or cortical granules, suggesting that the increase in calcium ions induces the cortical change.
Periodic changes in intracellular calcium ions accompanying cell cycle were recorded in fertilized medaka and sea urchin eggs injected with aequorin. In both eggs, intracellular calcium ion concentration attained a minimum during cleavage.
Transient and localized increases in intracellular calcium ions accoumpanying amoevoid movement and protoplasmic streaming in slime mold were recorded using aequorin as a probe.
Changes in intracellular calcium concentration were also recorded using fura 2, a fluorescent probe for calcium ions.
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