Laser-Excited High-Sensitice Fluorescence Microscope System for Biological Cells using High-Speed Multi-Image Processing
| Project/Area Number |
59850067
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
計測・制御工学
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| Research Institution | Tohoku University |
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Principal Investigator |
INABA Humio 東北大学, 電通研, 教授 (90006213)
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| Project Period (FY) |
1984 – 1985
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| Project Status |
Completed (Fiscal Year 1985)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥18,200,000)
Fiscal Year 1985: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1984: ¥10,700,000 (Direct Cost: ¥10,700,000)
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| Keywords | Laser / Single Biologica Cell / Fluorescence Microscope / High-Speed Multi-Image Processing / 3-Dimensional Intensity Display / 螢光寿命測定 |
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| Research Abstract |
This project aimed to the new development of high-sensitive fluorescence microscope system, which enables us to measure quntitatively fluorescence or scattering intensity distribution in a single viological cell utilizing optoelectronic technique including lasers and multiple image processing methods.
At first, We developes a laser-excited fluorescence microscope systen using an Ar laser for excitation, a SIT camera for sensitive detection and an image processor for digital processing of image signals. It was proved that this system can detect the fluorescence distribution from biological cell containing some organic dyes and analyze quantitatively the image signal from the SIT camara. Based on the results and understandings derived in the above study, we further improved the system employing an image intensifier and a new image processor to realize excellent high-sensitivity as well as better spatial and temporal resolutions. Thus, very weak fluorescence intensity distributions from se
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veral kinds of cells containing clinically interesting organic dyes were measured and displayed three-dimensionally with this system, which indicate accumulated portions of these dyes with in the cells. Temporal changes in fluorescence intensity distribution, which were mainly caused by the photochemical effect were also detected quatitatively in a short time interval less than 34 sec.
Moreover, a laser-excited high-speed, time-resolved fluorescrnce mivroscope system was newly developed to investigate fluorescence decays from various dyes in biological cells in nanosecond time scale. Using this system incorporating an UV pulsed <N_2> laser, we performed characteristics measurement and comparison of photosensitizing hematoporphyrin species and pheophorbide a in cultured cancer cells and solutions for the first time and their different behaviors were discussed.
In summary, we have succeeded in constructing a novel type of laser-excited high-speed fluorescnce microscope system for single biological cells using highspeed multi-image processing technique. The system and the results achieved by this Research Project should be useful and valuable in the fields of medicine, lifescience and viotechnology and their practical applications are expected in the near future. Less
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Report
(1 results)
Research Products
(10 results)
