| Project/Area Number |
59870001
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kyoto University |
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Principal Investigator |
OGAWA Kazuo Prof., Faculty of Medicine, Kyoto Univ., 医学部, 教授 (20077556)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Takao Associate Prof., Tottori Univ., School of Medicine, 医学部, 助教授 (20116312)
SAKAI Masahiro Instructor, Faculty of Medicine, Kyoto Univ., 医学部, 助手 (40183363)
NODA Toru Instructor, Faculty of Medicine, Kyoto Univ., 医学部, 助手 (50156204)
FUJIMOTO Kazushi Lecturer, Faculty of Medicine, Kyoto Univ., 医学部, 講師 (50159125)
FUJIMOTO Toyoshi Associate Prof., Faculty of Medicine, Kyoto Univ., 医学部, 助教授 (50115929)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥31,500,000 (Direct Cost: ¥31,500,000)
Fiscal Year 1986: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1985: ¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1984: ¥21,300,000 (Direct Cost: ¥21,300,000)
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| Keywords | Cathodoluminescence / Vitamin A / Three half ellipsoidal mirrors / Itocells / Adrenocortical zona fasciculata cells / Simple cryo-SEM method / カラー蛍光電子顕微鏡 / カソードルミネッセンス(CL) / 副腎皮質 / ステロイドホルモン / 螢光電子顕微鏡(CEM) |
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| Research Abstract |
In 1984, the intensities of cathodoluminescence (CL) emitting from fluorochromes, dyes, metals, biological substances and other materials were examined under an Akashi scanning electron microscope (SEM) ISI-DS130 equipped with a simple CL detector. Some salts of rare earth metals and some substances related to vitamin A (Vit A) metabolism and steroid hormone biosynthesis were proved to emit strong CL.
In 1985, the bottom stage of DS130 was modified with a half ellipsoidal mirror, photomultiplier tube (PMT) and monochrometer, so that precise CL images and CL spectra were able to obtain. CL images and spectra of Ito cells of Vit A loaded rat liver and zona fasciculata cells of rat adrenal cortex were observed and analyzed by means of the simple cryo-SEM method as a specimen preparation method for CL observation. They showed strong CL from their lipid droplets.
In 1986, the top stage of DS130 was remodeled into a color CL electron microscope (a color CEM). A newly shaped mirror, combining three half ellipsoidal mirrors, was induced, so that the detected CL photons could be amplified in red, green and blue zones separately. The specimen holder was totally changed in order to maintain the specimen temperature down to the liquid nitrogen temperature. Color CL images and CL spectra analysis of biological materials were turned out to be a useful method of identifying the unknown substances and displaying them in situ. Vit A esters were proved to emit CL from the lipid droplets of Ito cells under the color CEM.
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