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Automated high-performance apparatus for quantitative immunoblot analysis: Manufacture trial and its application to cell biochemistry

Research Project

Project/Area Number 59870011
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionKyoto University

Principal Investigator

MURACHI Takashi  Kyoto University, Faculty of Medicine, Professor, 医学部, 教授 (10089104)

Co-Investigator(Kenkyū-buntansha) NAKASE Yuzo  Tateisi Institute of Life Sciences, Laboratory Manager, ライフサイエンス研究所, 室長
KOBAYASHI Shigeki  Tateisi Institute of Life Sciences, Director, ライフサイエンス研究所, 所長
KANNAGI Reiji  Kyoto University, Faculty of Medicine, Lecturer, 医学部, 講師 (80161389)
TOTANI Masayuki  Kyoto University, Faculty of Medicine, Lecturer, 医学部, 講師 (70163988)
Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥17,300,000 (Direct Cost: ¥17,300,000)
Fiscal Year 1986: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1985: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1984: ¥11,600,000 (Direct Cost: ¥11,600,000)
Keywordsprotein / qualitative analysis / microquantitative determination / densitometer / data processing / immunoblotting / multiple staining / 細胞内成分分析 / イムノブロット / ウエスタンブロット / 細胞生化学
Research Abstract

1. Establishment of fundamental conditions for quantitative determination of immunoblots (1984). (1) A linear relationship in the range of 5 ng to 10 <mu> g of proteins was established using peroxidase. (2) High speed data processing enabled rapid scanning (3 cm/sec) and 1 M byte memory storage sufficed quantitative analysis.
2. Development of a testing machine and the fundamental experiment for applications to intracellular analysis (1985). Concentration vs. absorbance was linear over the range of optical density of 0 to 2.2 and the best sensitivity was obtained.
3. The completion of a testing machine and practical studies on intracellular analysis (1986). Biochemical and immunohistochemical distribution studies in various tissues with regard to calpain and calpastatin were advanced, and quantitative variation of these proteins was estimated by immunoblotting.

Report

(3 results)
  • 1986 Annual Research Report   Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] HATANAKA Michiyo: Biochemistry. 23. 3272-3276 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 信原美子: 日本臨床化学会年会記録. 25. 90 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 山肩葉子: 臨床病理. 33(補). 177 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 高野恵美子: 生化学. 57. 772 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 村地孝: 細胞工学. 15. 67-74 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 村地孝: 病態生理. 6. 133-139 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] MURACHI Takashi: "The Biological Role of Proteinases and Their Inhibitors in Skin." University of Tokyo Press, 10 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] HATANAKA, Michiyo: "Evidence for membrane-associated calpain I in human erythrocytes. Detection by an immunoelectrophoretic blotting method using monospecific antibody." Biochemistry. 23. 3272-3276 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] NOBUHARA, Yoshiko: "Protein determination by immunoblotting" Proceedings of the 25th Annual Meeting of Japan Society of Clinical Chemistry. 25. 90 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] YAMAGATA, Yoko: "Two cases of Factor XIII deficiency: Activity assay and immunological studies using Western blotting" Rinshobyori. 33. 177 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] MURACHI, Takashi: "Immunohistology of calpains" Saibokogaku. 15. 67-74 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] MURACHI, Takashi: "Trial manufacture and its application of automated apparatus for quantitative immunoblot analysis" Byotaiseiri. 6. 133-139 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] MURACHI, Takashi: University of Tokyo Press. The Biological Role of Proteinases and Their Inhibitors in Skin, 10 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] HATANAKA Michiyo: Biochemistry. 23. 3272-3276 (1984)

    • Related Report
      1986 Annual Research Report
  • [Publications] 信原美子: 日本臨床化学会年会記録. 25. 90 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 山肩葉子: 臨床病理. 33(補). 177 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 高野恵美子: 生化学. 57. 772 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 村地孝: 細胞工学. 15. 67-74 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 村地孝: 病態生理. 6. 133-139 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] MURACHI Takashi: "The Biological Role of Rroteinases and Their Inhibitors in Skin." University of Tokyo Press, 10 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 日本臨床化学会年会要旨集. .53 (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Investigative Ophthalmolgy and Visual Science. 26. P.953 (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Biochemistry International. 10. P.189 (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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