• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Developmental study of laser scan fluorescence microscope for objective diagnosis and screening of cancer cells

Research Project

Project/Area Number 59870016
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionKyoto Prefectural University of Medicine, School of Medicine

Principal Investigator

FUJITA Setsuya  Department of Pathology, Kyoto prefectural Univ. Medicine, 医学部, 教授 (00079716)

Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥18,100,000 (Direct Cost: ¥18,100,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1984: ¥4,400,000 (Direct Cost: ¥4,400,000)
Keywordslaser microscope / flurorescence microscopy / cytofluorometry of DNA / cancer cell diagnosis / screening of cancer cells / 機器試作 / レーザスキャン顕微鏡 / 共進点顕微鏡 / コンピュータ駆動ステージ
Research Abstract

It is important to introduce objective parameters into diagnosis of human cancer cells in pathological specimens. The reason is two-fold. One is to increase reliability of the diagnosis and the other is to facilitate future introduction of mechanical means into screening of cancer patients.
We have investigated significance of nuclear DNA determination and quantitative morphological analysis of cells in objective identification of cancer cells. In the present study, we attempted to develop laser scan fluorescence microscope to realize simultaneous determination of DNA quantity and morphological parameters that are of great help for pathologists in objective diagnosis of cancer cells.
In cooperation of Research Engeneers of the Olympus Co., we constructed survey instruments of the laser microscope (LSM-1 and-2) and tested optic system, mechanism of scanning of laser beam, how to take data, how to process the data in computer, and in 1960 we ordered LSM-G (the third machine) to the Olympus.
In 1961, we connect this LSM-G with a 32 bit desk top computer(HP9520) using HP-IB interface bus and developed a software "IMAG-9" to drive this system most efficiently. As the result, we found that;1. resolution is dramatically improved due to good quality of the laser beam and small size of the beam, 2. reliable quatitative data can be obtained, and 3. using confocal system, microtomography can be realized. These advantages will bring remarkable progress in cancer diagnosis.

Report

(2 results)
  • 1986 Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 藤田晢也,高松哲郎: 病態生理. 6. 207-214 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T.Takamatsu;S.Fujita;ed.by S.Ishizaka: Science on Form KTK Scientific and Reidel,Dordrecht. 1. 223-242 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hattori,T;Sugihra,H;Fukuda,M;Hamada,S;Fujita,S.: Japanese J.Cancer Research. 77. 276-281 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hattori,T;Hosokawa,Y;Sugihra,H;Fukuda,M;Fujita,S.: Pathology,Research and Practice. 180. 615-618 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Misawa,S;T.Abe;Takamatus,T;Fujita,S;T〓sta,J.R.: Japanese J.Human Genetics. 31. 297-307 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohtaki,M;Fujita,S;Hayakawa,N;Kurihara,M;Munaka,M.: Japanese J.Clin.Oncology. 15. 325-343 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 藤田晢也: "病理マニュアル。第4巻。顕微測光法の技術と定量的組織化学への応用" 朝倉書店, 325-354 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 藤田晢也: "病理学大系9A,腫瘍の増殖と生長" 中山書店, 70-111 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Fujita,S. & Takamatsu,T.: "Laser scanning fluorescence microscope and its possible application in biology and medicine" Medicina philosophica 6, 207-214 (1987). 6. 207-214 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Takamatsu,T. & Fujita,S.: "Color image analyzer and its application to quantitative biology" Science on Form (edited by Ishizaka,S.). 1. 223-242 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hattori,T.,Sugihara,H.,Fukuda,M. & Fujita,S.: "DNA ploidy patterns of minute carcinoma in the stomach" Japan J Cancer Research (Gann). 77. 276-281 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hattori,T.,Hosokawa,Y.,Sugihara,H.,Fukuda,M. & Fujita,S.: "DNA content of diffusely infiltrative carcinoma in the stomach" Path. Res. Pract.180. 615-618 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Misawa,S., Abe,T., Takamatsu,T., Fujita,S. & Testa, J.R.: "Determination of chromosomal area of double minute chromosomes and a homogeneously staining region in HL-60 leukemia cells by the use of color image analyzer." Japn J. Human Genet.31. 297-307 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Fujita,S.: Asakura,Tokyo. Pathology Manual, vol. 4 "Techniques of microphotometry and its application to quantitative histochemistry", 325-354 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Acta Histo.Cytochem.19-2. (1986)

    • Related Report
      1985 Annual Research Report
  • [Publications] Proc.ISSF. 1-1. (1985)

    • Related Report
      1985 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi