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Specific elimination of anti-DNA antibody-producing cells in systemic lupus erythematosus.

Research Project

Project/Area Number 59870026
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionTohoku University

Principal Investigator

SASAKI Takeshi  東北大学, 医, 助手 (50110656)

Project Period (FY) 1984 – 1985
Project Status Completed (Fiscal Year 1985)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1985: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1984: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsSLE / anti-DNA antibody / 抗イディオタイプ抗体
Research Abstract

The specific manipulation of anti-DNA antibody production is one of the prefered treatment of systemic lupus erythematosus (SLE). For this purpose, we induced DNE-specific tolerance in vitro using DNA-Dglutamic acid-D-lysine(DNA-D-GL).
The addition of the tolerogen reduced markedly the in vitro production of spontaneously occuring anti-ds and/or anti-ss DNA antibodies by SLE lymphocytes. The unresponsiveness was DNA-specific.
We also used monoclonal anti-idiotypic (Id) antibodies which were conjugated with neocarzinostatin (NCS), a cytotoxic agent. Human B cell clones, which produce anti-DNA autoantibodies, were killed by by in vitro treatment with anti-Id-NCS conjugates, while clones expressing an Id with irrelevant specificity were unaffected. Anti-Id-NCS also specifically suppressed anti-DNA autoantibodyproductin in vitro by human lymphocytes.
This approach will have a significant advantages in aborting clones, and will result in a potent therapeutic treatment for SLE.

Report

(1 results)
  • 1985 Final Research Report Summary

Research Products

(10 results)

All Other

All Publications

  • [Publications] J.Immunol.Methods. 72-157. (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Clin.Immunology. 5-246. (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Clin.Investigation. 76-1314. (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Immunology. 13-1241. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Clin.Investigation. 77. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Immunol.Methods. 72:157. (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Clin.Immunology. 5:246. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Clin.Invest.76:1314. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Immunol.136:1241. (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary
  • [Publications] J.Clin..Invest.77. (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1985 Final Research Report Summary

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Published: 1987-03-30   Modified: 2016-04-21  

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