| Project/Area Number |
59870029
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka University |
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Principal Investigator |
KAMADA Takenobu Osaka University Medical School, 医学部, 教授 (80028399)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANO Sunao Osaka University Medical School, 医学部, 助手 (60133138)
SATO Nobuhiro Osaka University Medical School, 医学部, 講師 (90028358)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1985: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1984: ¥9,700,000 (Direct Cost: ¥9,700,000)
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| Keywords | Microcirculation / Infra-red light / In vivo microscope / Image analyzing system / 電子内視鏡 / レーザー / 三次元解析 / 微小癌 / 酸素 / 肝臓消化管粘膜 |
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| Research Abstract |
Microcirculatory disturbance is emphasized to play an important role in the pathogenesis and development of various diseases. In order to investigate the organ microcirculation in three dimensions, we developed an in vivo microscopic system using SIT camera and image analyzer. A method for determining the RBC velocity in hepatic sinusoids and gastric mucosal capillaries was developed with dual slit photometry. Furthermore, fluorescein isothiocyanate(FITC) was used to detect the transport from the sinusoids to the bile canaliculi through hepatocytes. In the C <Cl_4> -damaged rat liver, RBC flow velocity decreased with WBC sticking to the sinusoid walls. FITC transport from sinusoid to bile canaliculi was significantly delayed.
In the gastric mucosal capillary, RBC flow velocity also showed the heterogeneity in normal rats. Various agents such as indomethacin and ethanol caused the decrease of RBC flow velocity, depending on the concentration of the drugs. More than 20% ethanol caused hem
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ostasis in the mucosal layer, while in the submucosal layer, the blood volume and hemoglobin oxygenation increased which suggests a presence of arterio-venular shunting in the submucosal layer.
A system for analyzing the gastric mucosal hemodynamics in real time, was developed using electronic endoscope. The results showed a marked microheterogeneity in blood flowing under hemorrhagic shock. This microheterogeneity had a relation with the localization of gastric ulcer. Also, a system for analyzing the vasculature in the deeper layer of human stomach was developed using electronic endoscope with infra-red light source. At the cancerous region, the abnormal vasculature pattern was clearly detected by this system.
In conclusion, the systems analyzing the organ microcirculation were developed using in vivo microscope with video processors and electronic videoendoscope with infra-red light. The microheterogeneity of blood flowing in hepatic sinusoids and gastric mucosal capillaries were indicated in damaged states and in cancer state. Less
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