| Budget Amount *help |
¥13,200,000 (Direct Cost: ¥13,200,000)
Fiscal Year 1985: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1984: ¥11,600,000 (Direct Cost: ¥11,600,000)
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| Research Abstract |
The mobility of water in the bone is determined by its interaction with macromolecules such as collagen proteoglycan and hydroxyapatite around the water molecules. Therefore, 1H-NMR relaxation times (T1,T2) of water in the bone, which indicate the mobility of water, might represent a microstructure of the tissue. On the other hand, water and lipid contents of the bone can be measured by 1H-NMR. Not only the microstructure, but also water and lipid contents of the bone might show some changes in its various pathological states. This project was carried out in order to analyze various pathological states of the bone by using 1H-NMR.
The experimental system was developed. The analog signals of 1H-NMR spectrometer were fed to transient memory system that was interfaced to micro-computer for on-line system control,data collection and analysis. The softwear for the system was designed to fit for the analysis of the bone pathology. The newly developed experimental system was applied to the glycosaminoglycan solution, to the cartilage, and to the bone.
Using this experimental system, the 1H-NMR signal of the bone which have very short relaxation time could be measured. The signal including two different relaxation times could be separated and analyzed satisfactorily. The glycosaminoglycan solution and the cartilage had a single component of T1 or T2 derived from water, and the relaxation times (T1,T2) were good indicators of the interaction between water and macromolecules. The cortical bone had a single component of T1 from water, the cancellous bone had two components: the long T1 from water, and the short T1 from lipid. The T1 from water in the bone was well correlative with its water content. When the cortical and cancellous bones had the same content of water, T1 was, however, always longer in the former than in the latter. T2 of the bone showed no special tendency.
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