• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Standarization of assay methods of endogenous prostaglandins and leukotrienes

Research Project

Project/Area Number 59870087
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionKitasato University School of Medicine

Principal Investigator

KATORI Makoto  Kitasato University School of Medicine Professor, 医学部, 教授 (50050365)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Shozo  Tokushima University School of Medicine Professor and four other memebers., 医学部, 教授 (50025607)
MUROTA Sei-itsu  Tokyo Medical and Dental University School of Dentistry Professor, 歯学部, 教授 (50072989)
TSURUFUJI Susumu  Tohoku University School of Pharmacy Professor, 薬学部, 教授 (40012596)
TADA Michihiko  Osaka University School of Medicine Professor, 医学部, 教授 (90093434)
SATOH Kazuo  Saitama Medical School, Saitama Medical Center Professor, 教授 (80010180)
Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥8,200,000 (Direct Cost: ¥8,200,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1984: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsAssay methods for prostaglandins / Elimination of disturbing substances for assay / Assay for 11-dehydro-Thromboxane <B_2> / leukotriene / 12-HETE / 6-keto-PG【F_(1α)】 / 11-dehydro-thromboxane【B_2】 / leukotriene【B_4】 / leukotriene【C_4】 / leukotriene【D_4】 / 前処理法 / 放射免疫測定法 / ガスクロマトグラフィ質量分析法
Research Abstract

This project team, composed of speakers of assay workshop in Kyoto Prostaglandin (PG) Conference, 1984, has focused the research on the following items, according to the conclusion of the workshop.
1. Establishment of clean-up method: 6-Keto- <PGF_(la)> in a test sample of human plasma containing a known amount (1 ng) was measured as a blind sample by own method of the team members (RIA, EIA, GC-MS with or without cleaning-up) to compare cleaning method. Variation of the values was rather small, but the following criticisms were made: (1) Sep-Pak C18, which was used by most members for separation of PGs, releases by organic solvent materials, which disturb RIA and EIA. (2) Thus, Sep-Pak C18 should be used in combination with HPLC or TLC for elimination of impure substances. (3) Purity of radio-labelled compounds for testing recovery as well as a difference between calibration curves of the standard also caused variation of the values.
2. Development of enzyme-immunoassay of 11-dehydro- <TXB_2> (DH- <TXB_2> ): Enzyme-immuno-assay of DH- <TXB_2> , a metabolite of <TXB_2> , has been developed, as <TXB_2> is generated artificially. Determination with the present kit revealed that an increase in DH- <TXB_2> values during blood collection was not observed and duration of blood concentration of DH- <TXB_2> was longer than that of <TXB_2> . DH- <TXB_2> value in urine from patients of essential hypertension was well correlated to that of <TXB_2> , but TXA synthetase inhibitor attenuated DH- <TXB_2> value in rat urine only slightly.
3. Leukotriene (LT) assay method: Cleaning-up must be made before using commercial RIA kits; acidification of samples at pH 3.5, followed by separation of LTs by Sep-Pak C18, enabled us to determine <LTB_4> and immunoreactive LTs without difficulty. It should be noticeable that 12-HETE on HPLC co-migrates with that of impure materials from ethanol, indicating that caution should be taken when 12-HETE is detected.

Report

(2 results)
  • 1986 Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] O.Ishihara: PGs. Lts. Med.24. 93-102 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Ohmori: Japanese Circulation Journal. 51. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] N.Hirasawa: Br.J.Pharmac.87. 151-156 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T.Yoshimoto: Biochem.Biophys.Acta. 877. 141-150 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Sato: Hypertension. 8. 762-771 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Sato: Agents and Actions. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] H.Matsuda: J.Chromatogr.374. 347-353 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T.Kuzuya: Cardiovasc.Res.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 佐藤牧人: 臨床検査 MooK. 24. 97-114 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] K.Muryobayashi: Prostaglandins.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Katori,et al.,Eds.O.Hayaishi;S.Yamamoto: "Seven articles in"Assay--Utility and Difficulty"in Advances in Prostagalndin,Thromboxane, and Leukotriene Research." Raven Press, 21-102 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 山本尚三,鹿取信 編: "プロスタグランジン研究法(上,下)" 東京化学同人, 250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 森田育男,室田誠逸,横田一成: "続 生化学実験講座3.膜脂質と血漿リポタンパク質下.18プロスタグランジン関連物質の分析法" 東京化学同人, 756 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T.Tonai: F. Shono: K Yokota: H. Miyazaki: K. Yamashita: K. Tanaka: T. Isono: N. Hirasawa: A. Kudo: "Enzyme immunoassay of 6-keto- <PGF_(la)> in a solid phase." "A solid-phase enzyme immunoassay of thromboxane <B_2> ." "Thromboxane <B_2> ." "Microdetermination of prostaglandins and thromboxane <B_2> by gas chromatography-negative ion chemical ionization mass spectrometry." "Quantitation of prostaglandins by gas chromatography--metastable ion monitoring." "Roles of leukotrienes in two rat allergic inflammatory models: IgE-mediated and IgG-antigen complex-induced pleurisies." "Measurement of immunoreactive leukotriene <C_4> in blood of asthmatic children." "Vascular permeability responses and the role of prostaglandin <E_2> in an experimental allergic inflammation of air pouch type in rats." "Urinary excretion of <TXB_2> after angiotensin converting enzyme inhibition in hypertensive patients." Biochim. Biophys. Acta. J. Biochem.Methods of Enzymatic Analysis.J. Chromatogr.J. Pharmacobio. Dyn.Prostaglandins. Biochem. Biophys. Res. Commun.Br. J. Pharmac.Prostaglandins, Leukotrienes Med.836. 98. 8. 289. 9. 31. 130. 87. 21. 335-343 69-77 50-59 249 70 67-84 486-492 151-156 77-86 (1985)(1985)(1985)(1984)(1986)(1986)(1985)(1986)(1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] H. Matsuda: Negative ion chemical ionization mass spectrometry of eicosanoids and its application to <PGI_2> synthesis., J. Chromatogr. 374, 347-353 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M. Katori; six authors Eds. O. Hayaishi; S. Yamamoto: Raven Press. Seven articles in "Assay--Utility and Difficulty" in Advances in Prostagalndin, Thromboxane,and Leukotirene Research, 21-102 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohuchi: Europ J.Pharmacol.117. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Isono: Biochem.Biophys.Res.Comm.130. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Matsuda: J.Chromatogr.(1986)

    • Related Report
      1985 Annual Research Report
  • [Publications] Tanaka: Prostaglandins. (1986)

    • Related Report
      1985 Annual Research Report
  • [Publications] Ueno: Prostaglandins. (1986)

    • Related Report
      1985 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi