| Project/Area Number |
59880010
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
1984 – 1985
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| Project Status |
Completed (Fiscal Year 1985)
|
| Budget Amount *help |
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1984: ¥7,300,000 (Direct Cost: ¥7,300,000)
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| Keywords | HPLC, Sugar analysis / 糖組成分析 / 螢光分析 / 微量糖分析法 |
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| Research Abstract |
Highly sensitive analysis of component sugars of glycoproteins has been developed. The processes of the method are hydrolysis of a sample with 2 M HC1-2 M trifluoroacetic acid (100゜C, 6 h), N-acetylation, pyridylamination of sugars, desalting by HPLC using a TSK-GEL G2000PW column, and chromatographic determination of pyridylamino derivatives of sugars by HPLC using a UltrasphereODS column. The method allowed the determination of 0.01-10 nmol of sugars. About 0.1 nmol of a sample can be analyzed with satisfactory results. Contaminating glucose peak on chromatogram could be reduced by burnning test tubes used for hydrolysis and by using freshly distilled water. Automation of the analysis was achieved by using an automatic injector (Waters 710B) and a fraction collector (FRAC-100. Pharmacia).
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